สืบค้นงานวิจัย
Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand
Pattara Wongjaroen - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Pattara Wongjaroen
บทคัดย่อ (EN): Zika virus is the important tropical disease which has big 2016 outbreak in South America and Asia. Zika virus infection diagnosis needs laboratory testing because of similar symptoms to dengue and chikungunya infections. The most useful detection method for Zika virus is real time RT-PCR. Because of the large amount of samples in 2016 Zika outbreak in Thailand, the original testing protocol is not suitable due to expensiveness and takes much time. This study aimed to develop the cost and time saving Zika virus detection method for using in Thailand. Real time RT-PCR for Zika virus detection was developed based on US-CDC’s method with modification. Viral RNA was extracted from 150 ml of sample then tested simultaneously with two sets of primer/probe targeting E and NS2b gene of Zika virus. The developed method can detect Thai isolate Zika virus spiked in urine and serum at least 0.15 PFU/ml. No false positive result occurred with Dengue, Chikungunya, Japanese encephalitis and West Nile viruses. The developed method showed satisfactory result with good limit of detection and no false positive with other viruses. Cost and time consuming was at 25% and 50% from the original method respectively. At Thai NIH, more than 10,000 samples had been tested with this method since 2016.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=626
เผยแพร่โดย (EN): National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
คำสำคัญ (EN): Urine
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand
Pattara Wongjaroen
National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
ไม่ระบุวันที่เผยแพร่
Development of Zika virus detection method by real time RT-PCR for using in Thailand Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Development and evaluation of multiplex real-time RT-PCR for Dengue, Chikungunya and Zika virus detection in Thailand Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method Development of Mayaro virus detection by real-time RT-PCR and retrospective surveillance of incident in Thailand during 2016-2019 Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand
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