คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Nitchakarn Noranate

เผยแพร่โดย

1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

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งานวิจัยที่แนะนำ The use of green fluorescent protein-tagged virus-like particles as a tracer in the early phase of chikungunya infection Characterization of Chikungunya Virus-Like Particles Vertical transmission of Indian Ocean Lineage of chikungunya virus in Aedes aegypti and Aedes albopictus mosquitoes Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Chemical composition and mosquitocidal activity of Cymbopogon citratus essential oil against Aedes aegypti from dengue and chikungunya risk areas Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus Monitoring arbovirus in Thailand: Surveillance of dengue, chikungunya and zika virus, with a focus on coinfections Characterization of an unusual DS-1-like G8P[8] rotavirus strain from Japan in 2017: evolution of emerging DS-1-like G8P[8] strains through reassortment Anti-Chikungunya Virus Monoclonal Antibody That Inhibits Viral Fusion and Release Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus

คัดลอก URL

ชื่อเรื่อง (EN):	Characterization of chikungunya virus-like particles
ผู้แต่ง / หัวหน้าโครงการ (EN):	Nitchakarn Noranate
บทคัดย่อ (EN):	Chikungunya virus (CHIKV) is becoming a global concern due to the increasing number of outbreaks throughout the world and the absence of any CHIKV-specific vaccine or treatment. Virus-like particles (VLPs) are multistructured proteins that mimic the organization and conformation of native viruses but lack the viral genome. They are noninfectious and potentially safer vaccine candidates. Recent studies demonstrated that the yield of CHIKV VLPs varies depending on the strains, despite the 95% amino acid similarity of the strains. This might be due to the codon usage, since protein expression is differently controlled by different organisms. We optimized the region encoding CHIKV structural proteins, C-E3-E2-6k-E1, inserted it into a mammalian expression vector, and used the resulting construct to transfect 293 cells. We detected 50-kDa proteins corresponding to E1 and/or E2 in the cell lysate and the supernatant. Transmission electron microscopy revealed spherical particles with a 50- to 60-nm diameter in the supernatant that resembled the native CHIKV virions. The buoyant density of the VLPs was 1.23 g/mL, and the yield was 20 µg purified VLPs per 108 cells. The VLPs aggregated when mixed with convalescent sera from chikungunya patients, indicating that their antigenicity is similar to that of native CHIKV. Antibodies elicited with the VLPs were capable of detecting native CHIKV, demonstrating that the VLPs retain immunogenicity similar to that of the native virion. These results indicated that CHIKV VLPs are morphologically, antigenically, and immunologically similar to the native CHIKV, suggesting that they have potential for use in chikungunya vaccines.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=464
เผยแพร่โดย (EN):	1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi, Thailand 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
คำสำคัญ (EN):	chikungunya virus-like particles
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science