สืบค้นงานวิจัย
Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment
Jariyapong P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment
ผู้แต่ง / หัวหน้าโครงการ (EN): Jariyapong P.
บทคัดย่อ (EN): Macrobrachium rosenbergii nodavirus (MrNV) is usually accompanied by extra small virus (XSV) in natural outbreaks of white tail disease (WTD) in the giant river prawn Macrobrachium rosenbergii. Testing the virulence of MrNV alone has been problematic due to the difficulty in completely separating XSV from MrNV by viral purification steps from naturally infected shrimp. However, based on reports of natural M. rosenbergii specimens from WTD outbreak ponds that were positive for MrNV but negative for XSV led us to hypothesize that MrNV alone might cause WTD. To test this hypothesis, we prepared the two, complete genomic RNA fragments (RNA1 and RNA2) of MrNV from cDNA clones and used these to transfect Sf9 cells that subsequently showed cellular changes, including cell swelling, syncytial cell formation, and development of cytoplasmic inclusions within 72 h post-transfection. Replication of RNA1 and RNA2 increased in the transfected cells and transmission electron microscopy of the cell lysates revealed the presence of icosahedral viral-like particles that were 40–50 nm in diameter. When naïve Sf9 cells were inoculated with the cell lysate, the newly infected cells showed cellular changes and produced strong immunoreactivity against MrNV capsid protein indicating the infectious nature of the cell lysate. When the lysates were injected into the whiteleg shrimp Penaeus vannamei, MrNV RNA replication in the shrimp was followed by morality accompanied by typical MrNV lesions that gave possible positive immunohistochemical reactions for the MrNV capsid protein. Treatment of the Sf9 cells with azidothymidine triphosphate (AZT) prior to transfection significantly increased viral RNA synthesis and pathogenicity when compared with untreated, transfected cells. Using this model to produce infectious MrNV without XSV contamination proves that MrNV alone can be lethal to shrimp and it opens the way to further investigate the molecular basis of MrNV pathogenesis, and to develop antiviral strategy to control white tail disease. © 2017 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031498892&doi=10.1016%2fj.aquaculture.2017.10.008&partnerID=40&md5=1e9c6a1d233242c29e0138ce6b196626
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): White tip die-back phytoplasma
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Jariyapong P.. "Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment". ไม่ระบุหน่วยงาน. ม.ป.ป.

Jariyapong P.. "Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment
Jariyapong P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
The existence of kisspeptin-like peptides and effects on ovarian development and maturation in the giant freshwater prawn Macrobrachium rosenbergii Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus Distinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergii Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA Hematopoietic tissue of Macrobrachium rosenbergii plays dual roles as a source of hemocyte hematopoiesis and as a defensive mechanism against Macrobrachium rosenbergii nodavirus infection Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis ผลของสไปรูไลนา (Spirulina platensis) ต่ออัตราการรอดของกุ้งก้ามกราม (Macrobrachium rosenbergii ) ที่ติดเชื้อ Macrobrachium rosenbergii Nodavirus และ Extra small virus Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii
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