คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Benjawan Phetsuksiri

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand. 2 Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020. 3 Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 001-0020, Japan. 4 Section of Bacterial Infections, Research Collaboration Center for Emerging and Re-emerging Infectious Diseases, Nonthaburi, Thailand. 5 Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.

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งานวิจัยที่แนะนำ Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test การตรวจหาเชื้อมาลาเรียจากฟิล์มเลือดด้วยเทคนิค Real-time Nucleic Acid Sequence-based Amplification และ Molecular beacon The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสและเลปโตสไปโรสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis and leptosp Development of a rapid, simple method for detecting Naegleria fowleri visually in water samples by Loop-Mediated Isothermal Amplification (LAMP) Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DN Shewanella putrefaciens in cultured tilapia detected by a new calcein-loop-mediated isothermal amplification (Ca-LAMP) method

คัดลอก URL

ชื่อเรื่อง (EN):	Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis in Comparison with Immunochromatographic SD Bioline MPT64 Rapid® in a High Burden Setting
ผู้แต่ง / หัวหน้าโครงการ (EN):	Benjawan Phetsuksiri
บทคัดย่อ (EN):	Loop-mediated isothermal amplification (LAMP) was assessed for rapid identification of Mycobacterium tuberculosis complex (MTC) in comparison with an immunochromatographic test (ICT) using SD Bioline Ag MPT64 Rapid®. One hundred and fifty-one MGIT cultures positive for acid-fast bacilli were tested for MTC. DNA was extracted from a small portion of culture samples by heat lysis and subjected to LAMP analysis. Of these, 144 were positive and 5 were negative by both tests. One culture that was ICT negative but was LAMP positive was confirmed to have a mutation in the mpt64 gene. The agreement was 98.68% (95% confidence interval [CI]: 94.80–99.77), and the kappa value was 0.83% (95% CI: 0.59–1.00). Good correlation results suggested that LAMP assay is a reliable molecular test for rapid identification of MTC and is practical for use in resource-limited, high burden settings.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=549
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand. 2 Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020. 3 Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 001-0020, Japan. 4 Section of Bacterial Infections, Research Collaboration Center for Emerging and Re-emerging Infectious Diseases, Nonthaburi, Thailand. 5 Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
คำสำคัญ (EN):	Loop-Mediated Isothermal Amplification
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science