สืบค้นงานวิจัย
Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method
Pattara Wongjaroen - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method
ผู้แต่ง / หัวหน้าโครงการ (EN): Pattara Wongjaroen
บทคัดย่อ (EN): Introduction: Dengue, Chikungunya and Zika viruses are arthropod-borne viruses of which Aedes mosquito is a vector. Clinical symptoms caused by those viral infections are similar. Hence, laboratory diagnosis to differentiate each infectious agent is essential. Real-time RT-PCR is an assay of high sensitivity, high specificity and low cross-reaction as compared with serological tests. In addition, multiplex real-time RT-PCR can detect several genetic materials in one reaction. This study was, therefore, aimed to develop the multiplex real-time RT-PCR for detection of Dengue, Chikungunya and Zika viruses in suspected clinical specimens. Materials and methods: Specific primers and probes were designed and laboratory strains of Dengue1 (16007), Dengue2 (16681), Dengue3 (16562), Dengue4 (4328S), Chikungunya (Ross) and Zika (SV0127/14) viruses were used for protocol optimization and determination of limit of detection (LOD). Samples of Japanese encephalitis, Yellow fever and West Nile virus were tested for cross reactivity. A total of 140 known positive samples and 23 known negative samples for Dengue, Chikungunya and Zika viral infections were used to determine clinical sensitivity and specificity of the developed method. Those positive samples consisted of 80, 15 and 45 Dengue (20 for each serotype), Chikungunya and zika-infected specimens, respectively. Results: All laboratory strains were detected with the developed method. LOD for Dengue1 (16007), Dengue2 (16681), Dengue3 (16562), Dengue4 (4328S) viruses were approximately 0.46, 8, 8 and 17.2 FFU/ml, respectively. Additionally, LOD for Zika (SV0127/14) and Chikungunya (Ross) viruses were approximately 89 and 21 copies/ml, respectively. No cross-reactions to Japanese encephalitis, Yellow fever and West Nile viruses were found. Using the method developed, clinical sensitivity for detection of Dengue and Chikungunya viruses was 100%, while that for Zika virus was 95.56%. In addition, clinical specificity of this method was 100% for all three viruses. Furthermore, no false positive results were reported. Discussion: The developed method showed satisfactory results with acceptable sensitivity and specificity. Negative results found in some Zika-infected samples could possibly due to very low titer of virus in those specimens.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=670
เผยแพร่โดย (EN): National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
คำสำคัญ (EN): Real time RT-PCR
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Pattara Wongjaroen. "Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method". ไม่ระบุหน่วยงาน. ม.ป.ป.

Pattara Wongjaroen. "Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method
Pattara Wongjaroen
National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
ไม่ระบุวันที่เผยแพร่
Development of Zika virus detection method by real time RT-PCR for using in Thailand Development and validate method for zoonoses test kits Development and evaluation of multiplex real-time RT-PCR for Dengue, Chikungunya and Zika virus detection in Thailand Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand Antibody response to Zika and Dengue virus among the Zika realtime PCR positive cases in Thailand, 2016 ผลการแยกชนิดของระยะตัวเต็มวัยและไข่ของพยาธิเข็มหมุดโดยวิธี PCR ผลงานนำเสนอประชุมวิชาการกรมวิทยาศาสตร์การแพทย์ ครั้งที่ 23 เรื่อง ผลการแยกชนิดของระยะตัวเต็มวัยและไข่ของพยาธิเข็มหมุดโดยวิธี PCR Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay Characterization of Chikungunya Virus-Like Particles Zika Incidence among Patients with Suspected Dengue Fever (Preliminary Report)
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