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Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3))
Yulnawati Yusnizar - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3))
ผู้แต่ง / หัวหน้าโครงการ (EN): Yulnawati Yusnizar
หน่วยงานสังกัดผู้แต่ง (EN): Cibinong (Indonesia). Research Center for Biotechnology
บทคัดย่อ (EN): The objective of this research was to examine the effectivity of sugar palm juice as an alternative extender to cryopreserve buffalo. Moreover, we expected to obtain the best composition of sugar palm juice that can be used widely as an alternative extender for buffalo semen cryopreservation. Semen was collected from swamp buffalo bull using artificial vagina. Fresh, diluted, equilibrated, and frozen-thawed was evaluated for general parameters, i.e spermatozoa progressive motility (M), viability (V) and membrane integrity (MI). Fresh semen that had good quality was diluted up to 1.10**(8) sperm/ml in four different compositions of extenders, i.e lactose extender containing 20 percent egg yolk as control (C), 74 percent sugar palm juice + 20 percent egg yolk + 6 percent glycerol (SPG6), 73 percent sugar palm juice + 20 percent egg yolk + 7 percent glycerol (SPG7), and 72 percent sugar palm juice + 20 percent egg yolk + 8 percent glycerol (SPG8), respectively. Diluted-semen was equilibrated at 5 deg C for 4 h. It followed by frozen and stored in liquid nitrogen (-196 deg C). The results showed that the percentages of M, V, and MI of frozen-thawed spermatozoa in control (C) were 45.83, 59.37, and 57.83 percent), respectively. These results were significantly (P LT 0.05) higher than those parameters in SPG7 (30.83, 38.83, and 48.83 percent), SPG6 (18.33, 38.83, and 40.33 percent), and SPG8 (18.33, 39.17, and 39.33 percent). In conclusion, sugar palm juice might not be as ideal as control group in maintaining frozen-thawed swamp buffalo semen quality. But this inform us that in a very remote area where chemical or commercial extenders are not easy to find, sugar palm juice can be an alternative due to the fact that it did not affect the pregnancy rate of AI program. Moreover, the cryoprotectant (glycerol) concentration play important role in order to maintain the spermatozoa post thawed quality.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/402530
คำสำคัญ (EN): Indonesia
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Yulnawati Yusnizar. "Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Yulnawati Yusnizar. "Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3))
Yulnawati Yusnizar
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Cholesterol supplementation in extender improves quality of frozen-thawed Nili-Ravi buffalo bull semen (Buffalo Bulletin (Thailand),35(2)) Cryptosporidiosis in buffalo calves (Buffalo Bulletin,39(2)) Effect of antioxidant additives on freezability of buffalo spermatozoa (Buffalo Bulletin,39(3)) Cervical oesophageal obstruction in a she-buffalo (Buffalo Bulletin,39(4)) Morphological studies of cryopreserved Toda buffalo spermatozoa by CASA (Buffalo Bulletin (Thailand),36(2)) Determining hygienic protocols for swamp buffalo milking and food safety in Thailand (Buffalo Bulletin,39(4)) Cryopreservation induces capacitation-like changes of the swamp buffalo spermatozoa (Buffalo Bulletin (Thailand),36(1)) A note on successful treatment of toxocarosis in buffalo calves (Buffalo Bulletin,39(3)) Production of buffalo milk (Bubalus bubalis) in Brazil (Buffalo Bulletin,39(3)) Histochemical studies of liver in buffalo (Bubalus bubalis) (Buffalo Bulletin,39(2))
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