สืบค้นงานวิจัย
Performance of TaqMan array card to detect TB drug resistance on direct specimens
Banu S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Performance of TaqMan array card to detect TB drug resistance on direct specimens
ผู้แต่ง / หัวหน้าโครงการ (EN): Banu S.
บทคัดย่อ (EN): Culture based phenotypic drug susceptibility testing (DST) for Mycobacterium tuberculosis (TB) is time consuming therefore rapid genotypic methods are increasingly being utilized. We previously developed and evaluated on TB isolates a rapid genotypic TaqMan array card (TAC) that detects mutations in several resistance-associated genes using dozens of primer pairs, probes, and high resolution melt analysis, with >96% accuracy versus Sanger sequencing. In this study we examined the performance of TAC on sputum, comparing results between 71 paired sputum and TB isolates of which 62 were MDR-TB. We also adapted the TAC to include wild-type probes and broadened coverage for rpoB and gyrA mutations. TAC was 89% successful at detecting wild-type or mutations within inhA, katG, rpoB, eis, gyrA, rplC, and pncA on smear positive sputa and 33% successful on smear negative sputa. The overall accuracy of these detections as compared to the TAC results of the paired isolate was 95% ± 7 (average sensitivity 98% ± 3; specificity 92% ± 14). Accuracy of sputum TAC results versus phenotypic DST for isoniazid, rifampin, ofloxacin/moxifloxacin, and pyrazinamide was 85% ± 12. This was similar to that of the isolate TAC results (accuracy 88% ± 13), thus inaccuracies primarily reflected intrinsic genotypic-phenotypic discordance. The TAC is a rapid, modular, comprehensive, and accurate TB DST for the major first and second line TB drugs and could be used for supplemental testing of GeneXpert resistant smear positive sputum. © 2017 Banu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019131030&doi=10.1371%2fjournal.pone.0177167&partnerID=40&md5=16090ba41b13a7e499c2ce63bd8100b5
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Reverse Transcriptase Polymerase Chain Reaction
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Banu S.. "Performance of TaqMan array card to detect TB drug resistance on direct specimens". ไม่ระบุหน่วยงาน. ม.ป.ป.

Banu S.. "Performance of TaqMan array card to detect TB drug resistance on direct specimens". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Performance of TaqMan array card to detect TB drug resistance on direct specimens
Banu S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Use of dried blood spot specimens for in-house HIV drug resistance genotyping assay among infants in Thailand Prevalence of primary HIV drug resistance in Thailand detected by short reverse transcriptase genotypic resistance assay Genotypic antimicrobial resistance assays for use on E. Coli isolates and stool specimens Molecular assays for antimalarial drug resistance surveillance: A target product profile External Quality Assessment Scheme for HIV-1 Drug Resistance Genotyping in Thailand Chloroquine is grossly under dosed in young children with malaria: Implications for drug resistance Combinatorial Genetic Modeling of pfcrt-Mediated Drug Resistance Evolution in Plasmodium falciparum National Survey of Pretreatment HIV-Drug Resistance in Thai HIV-1-Infected Adults Risk factors that may be driving the emergence of drug resistance in tuberculosis patients treated in Yangon, Myanmar Pooled sequencing and rare variant association tests for identifying the determinants of emerging drug resistance in malaria parasites
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair