สืบค้นงานวิจัย
Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis
C. Charoenwut - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis
ผู้แต่ง / หัวหน้าโครงการ (EN): C. Charoenwut
บทคัดย่อ (EN): Trunk Phloem Necrosis (TPN) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantation. TPN is favored by accumulation of various stresses, leading to a rather abrupt, total and often irreversible cessation of latex flow. However, the very first initial molecular and cellular events leading to TPN remain unclear. To investigate the molecular mechanism of TPN outbreak, and look for specific molecular markers of this disease, the analysis of differentially expressed genes in TPN and healthy trees using a PCR-based suppression subtractive hybridization (SSH) technique had previously been performed. The mRNA isolated from TPN-affected trees, at the early stage of the disease, was used as “tester” and the mRNA isolated from healthy trees was used as “driver” and vice versa. After EST sequencing of our phloem SSH libraries, the differentially enriched cDNA fragments had been analyzed and identified using the EST-Data Base (ESTDB) software pipeline (Genetrop/IRD), including the NCBI blast program, and classified according to their functions (Kongsawadworakul et al., 2005). In this communication we report that macroarray analysis was performed to confirm the differential expression of those genes. From the macroarray data, some genes were confirmed to be highly up-regulated in the phloem of trees undergoing the TPN disease. Among them three unknown-function genes, showing the highest expression level in TPN trees, were selected as the best candidate genes : two “No hit” (unknw1 and unknw2) and one Stress and Disease Related Protein (Sdrp). Northern blot analysis confirmed high expression level of the unknw1 genes in the phloem at the bottom part of the trunk of necrotic (early stage of the disease) trees only, compared to healthy trees, in both the PB260 and GT1 rubber clones. These results were confirmed for all the 3 genes on more trees by semi-quantitative RT-PCR. We checked the expression pattern of these genes along the bottom part of the trunk of trees (PB260 and GT1 clones) developing early step of the disease (expansion of the inner phloem necrosis from the rootstock-scion junction towards the tapping cut). Semi-quantitative RT-PCR showed the highest expression of these 3 genes nearby the tapping cut, i.e. at the front of the extending disease, compared to the underneath areas already exhibiting severe necrotic symptoms. The tapped and untapped healthy trees did not significantly express these genes whatever the sampling area. High over expression of these genes was confirmed by qRT-PCR. The full-length cDNA clones of these unknown-function TPN molecular markers are presently under cloning, for further characterization.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): TPD
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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C. Charoenwut. "Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis". ไม่ระบุหน่วยงาน. ม.ป.ป.

C. Charoenwut. "Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis
C. Charoenwut
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Is impeded root growth related to the occurrence of rubber tree Trunk Phloem Necrosis (TPN)? Preliminary results from NE Thailand RUBBER TREE TRUNK PHLOEM NECROSIS IN NORTHEAST THAILAND: 1-INVESTIGATIONS ON SOIL HETEROGENEITIES AND DISEASE DISTRIBUTION POTENTIAL OF INCREASING THE PLANTING DENSITY OF RUBBER IN SRI LANKA AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE RUBBER TREE TRUNK PHLOEM NECROSIS (TPN) IN NORTHEAST THAILAND: 2, WITHIN-STAND CHARACTERISATION OF AFFECTED TREES STUDY ON THE POTENCY OF SERRATIA BACTERIA USED TO CONTROL WHITE ROOT DISEASE IN RUBBER RUBBER FACTORY EFFLUENTS TREATMENT WITH UASB –STABILIZATION POND- OXIDATION DITCH METHOD A FIRST PHYSIOLOGICAL ASSESSMENT OF LATEX CLONAL METABOLIC TYPOLOGY AND RUBBER YIELD POTENTIAL OF “CMS” RUBBER TREE CLONES Effect of Temperature Constraints on Photosynthesis of Rubber (Hevea brasiliensis) The Role of Myanmar Rubber Planters and Producers Association (MRPPA) in Natural Rubber Development and its Recent Activities
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