สืบค้นงานวิจัย
Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation
Phakdeedindan P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation
ผู้แต่ง / หัวหน้าโครงการ (EN): Phakdeedindan P.
บทคัดย่อ (EN): Stem cells are promising cell source for treatment of multiple diseases as well as myocardial infarction. Rabbit model has essentially used for cardiovascular diseases and regeneration but information on establishment of induced pluripotent stem cells (iPSCs) and differentiation potential is fairly limited. In addition, there is no report of cardiac differentiation from iPSCs in the rabbit model. In this study, we generated rabbit iPSCs by reprogramming rabbit fibroblasts using the 4 transcription factors (OCT3/4, SOX2, KLF4, and c-Myc). Three iPSC lines were established. The iPSCs from all cell lines expressed genes (OCT3/4, SOX2, KLF4 and NANOG) and proteins (alkaline phosphatase, OCT-3/4 and SSEA-4) essentially described for pluripotency (in vivo and in vitro differentiation). Furthermore, they also had ability to form embryoid body (EB) resulting in three-germ layer differentiation. However, ability of particular cell lines and cell numbers at seeding markedly influenced on EB formation and also their diameters. The cell density at 20,000 cells per EB was selected for cardiac differentiation. After plating, the EBs attached and cardiac-like beating areas were seen as soon as 11 days of culture. The differentiated cells expressed cardiac progenitor marker FLK1 (51 ± 1.48%) on day 5 and cardiac troponin-T protein (10.29 ± 1.37%) on day 14. Other cardiac marker genes (cardiac ryanodine receptors (RYR2), α-actinin and PECAM1) were also expressed. This study concluded that rabbit iPSCs remained their in vitro pluripotency with capability of differentiation into mature-phenotype cardiomyocytes. However, the efficiency of cardiac differentiation is still restricted. © 2019 Japanese Association for Laboratory Animal Science.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063514398&doi=10.1538%2fexpanim.18-0074&partnerID=40&md5=be239e3b6e3b94404431254036397cd6
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Stage-Specific Embryonic Antigens
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation
Phakdeedindan P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Generation of porcine induced-pluripotent stem cells from Sertoli cells A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs) In vitro production of functional immune cells derived from human hematopoietic stem cells Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages Computational identification of miRNAs that modulate the differentiation of mesenchymal stem cells to osteoblasts Therapeutic benefit of intra-articular administration of deciduous teeth stem cells in rabbit model of osteoarthritis EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS Ferrous and ferric differentially deteriorate proliferation and differentiation of osteoblast-like UMR-106 cells Enhancement of TNF-α induced antiproliferation in human cervical cancer cells by extracts from Thai medicinal plants Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells
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