สืบค้นงานวิจัย
Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products
Chewchinda S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products
ผู้แต่ง / หัวหน้าโครงการ (EN): Chewchinda S.
บทคัดย่อ (EN): A high performance thin layer chromatographic (HPTLC) method was developed and validated for simultaneous determination of rhein and aloe-emodin, major anthraquinone constituents, in S. alata leaves. The separation was performed on a silica gel 60 F254 HPTLC plate using ethyl acetate: methanol: water (100:17:10, v/v/v) as the development system. Validation of the analytical method for rhein and aloe-emodin promoted acceptable parameters. Good linearity in the range of 40-480 ng/band was obtained while intra-day and inter-day precisions were shown to be precise with relative standard deviations of less than 5%. The average percentage recoveries of rhein and aloe-emodin were 98.8% and 98.9%, suggesting acceptable accuracy. The content of rhein and aloe-emodin in S. alata leaves collected from 5 different provinces in Thailand analyzed by the validated HPTLC method were in the ranges of 0.098 ± 0.017 - 0.30 ± 0.02%, w/w, and 0.081 ± 0.0006 - 0.34 ± 0.0009 %, w/w, respectively. Five commercial products of S. alata tea available in the market were purchased and analyzed for rhein and aloe-emodin contents. The contents of rhein and aloe-emodin in the tea samples were in the ranges of 0.085 ± 0.004 - 0.23 ± 0.04%, w/w, and 0.096 ± 0.006 - 0.30 ± 0.01 %, w/w, respectively. The HPTLC method is rapid, reliable, sensitive and economical for routine analysis of rhein and aloe-emodin contents in S. alata leaf raw materials and its commercial products.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019104902&partnerID=40&md5=6f0e2830e80ab76f88e64ee700d173e4
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thailand
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Chewchinda S.. "Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chewchinda S.. "Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products
Chewchinda S.
มหาวิทยาลัยมหิดล
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