คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Wiphat Klayut

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand. 2 Department of Medical Technology, Maesot Hospital, Tak Province, Thailand.

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture Method verification of Isolated Chicken Eye Test Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis in Comparison with Immunochromatographic SD Bioline MPT64 Rapid® in a High Burden Setting ประสิทธิภาพของวิธี in-house loop-mediated isothermal amplification ในการตรวจหาเชื้อวัณโรคจากตัวอย่างเสมหะ เปรียบเทียบกับวิธี Xpert MTB/RIF Efficacy Evaluation Test 15min Rapid molecular detection of multidrug- resistant tuberculosis by PCR-nucleic acid lateral flow immunoassay ศึกษาเปรียบเทียบวิธีการหา Acid detergent fiber A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis Tuberculin skin test and QuantiFERON®-TB Gold In-tube Test for diagnosing latent tuberculosis infection among Thai healthcare workers Rapid detection of tuberculosis resistant to isoniazid and rifampicin by real-time PCR and melting curve analysis

คัดลอก URL

ชื่อเรื่อง (EN):	Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test
ผู้แต่ง / หัวหน้าโครงการ (EN):	Wiphat Klayut
บทคัดย่อ (EN):	Tuberculosis remains a major health problem. Detection of Mycobacterium tuberculosis from clini-cal specimens is an important process to diagnose of the disease. A molecular testing that provides an accurate and is a faster than standard bacteriological cultures is used to combine with the conventional methods. Xpert MTB/RIF, a molecular testing that is endorsed by World Health Organization uses an automated real-time PCR machine called “GeneXpert" to simultaneous detection of M. tuberculosis and its rifampicin resistance directly from clinical specimens which offers a high sensitivity and specificity. This research aimed to study performance of Xpert MTB/RIF in the direct detection of M. tuberculosis and its rifampicin resistance comparing to standard culture methods and rifampicin susceptibility testing by liquid media. One hundred and forty of decontaminated sputum from routine TB laboratory service were used. After culturing, a remaining sample was analyzed by Xpert MTB/RIF. It was found that the sensitivity and specificity of Xpert MTB/RIF were 88.55% (116/131) and 100.0% (979), respectively compared with TB culture, and 87.5% (7/8) and 97.18% (69/71), respectively compared with liquid susceptibility testing. Therefore, we concluded that Xpert MTB/RIF was suitable to use for detection of M. tuberculosis and its rifampicin resistance in clinical specimens which was highly sensitive and require less detecting time fewer than 2 hours.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=519
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand. 2 Department of Medical Technology, Maesot Hospital, Tak Province, Thailand.
คำสำคัญ (EN):	tuberculosis; rifampicin-resistant tuberculosis; Xpert MTB/RIF
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science