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Characterization of coding areas of SPGA11B gene in Murrah bulls (Buffalo Bulletin,41(2))
Richa Mishra - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Characterization of coding areas of SPGA11B gene in Murrah bulls (Buffalo Bulletin,41(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Richa Mishra
หน่วยงานสังกัดผู้แต่ง (EN): Uttar Pradesh (India)
บทคัดย่อ (EN): The SPAG11 gene is one of pivotal molecules in reproduction as it takes part in spermatozoa maturation, acquiring motility, capacitation, and egg-sperm interaction as well. The current study was aimed to characterize SPAG11 gene in Indian Murrah bulls through direct DNA sequencing approach. Genomic DNA from Murrah animals were isolated from 130 Murrah bulls and amplified using three sets of forward and reverse primers which were based on reference sequence (Genbank accession no. AC_000164.1) of Bos taurus covering entire coding region of SPAG11B gene. The PCR products of 563, 340 and 373, bp covering exons 1 to 3 were subjected to sequencing and subsequently ClustalW analysis revealed the substitution at 34 positions and a single stretch of 22 bp deletion in comparison to the Bos taurus reference sequence. Total seven novel SNPs were observed as two in the coding region and five in 5ˈUTR. However, only one of SNPs resulted in amino acid substitution viz. p.1279 arginine to tryptophan in translated protein in Murrah buffaloes. Sequence alignment and homology across species for the targeted nucleotide sequence of SPAG11 gene in Murrah bulls was done by nucleotide BLAST (NCBI) that showed maximum identity of 97 percent with mRNA of Bos taurus and Capra hircus followed by 96 percent homology with Bos indicus and Bison bison and 95 percent homology with Ovis aries and Bos mutus.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/418062
คำสำคัญ (EN): DNA sequencing Murrah bulls
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Richa Mishra. "Characterization of coding areas of SPGA11B gene in Murrah bulls (Buffalo Bulletin,41(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Richa Mishra. "Characterization of coding areas of SPGA11B gene in Murrah bulls (Buffalo Bulletin,41(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Characterization of coding areas of SPGA11B gene in Murrah bulls (Buffalo Bulletin,41(2))
Richa Mishra
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Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1)) Expression and structural variations of toll-like receptor 4 gene in buffalo and cattle (Buffalo Bulletin,41(1)) Histoarchitectural study of mammary alveoli on lactation, involution and pregnant stage in Murrah buffalo (Buffalo Bulletin,41(1)) Obstetrical management of Perosomus horridus fetal monster with brachynathism in a graded Murrah buffalo (Buffalo Bulletin,41(2)) Dicephalus fetal monster in a Murrah buffalo: A case report (Buffalo Bulletin,41(3)) Diagnosis and therapeutic management of oral tumours in Murrah buffaloes (Buffalo Bulletin,41(1))
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