สืบค้นงานวิจัย
Genetic polymorphism of aquaporin 7 gene and its association with semen quality in Surti bulls (Buffalo Bulletin (Thailand),37(2))
Beena Sinha - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Genetic polymorphism of aquaporin 7 gene and its association with semen quality in Surti bulls (Buffalo Bulletin (Thailand),37(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Beena Sinha
หน่วยงานสังกัดผู้แต่ง (EN): Karnal (India). Indian Council of Agricultural Research. Animal Genetics and Breeding Division
บทคัดย่อ (EN): The present study was carried out with the aim to characterise Aquaporin 7 (AQP7) gene and to investigate the association of genetic variants with semen quality in Surti bulls. A total of 21 Surti bulls maintained at Centralized Semen Collection Centre, Dharwad, Karnataka were included in the study. Single Nucleotide Polymorphisms (SNPs) in the coding region of AQP7 gene were identified through polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis and direct sequencing. A total of 14 different SSCP variants were found in exonic regions along with the exon-intron boundaries (amplicons) of AQP7 gene. Statistical analysis was done by SAS 9.3. Analysis of exons (amplicons) 1, 4, 5, 7 and 8 revealed two, three, four, three and two unique SSCP band patterns, respectively. SSCP variants of exon 1 were found to have significant (P LT 0.05) effect on Hypo-osmotic swelling reactivity in frozen semen. SSCP variants of exons 5 and 8 were found to have significant (P LT 0.01) effect on sperm viability in fresh semen and Post thaw motility, respectively. The study indicated high degree of genetic variability in AQP7 gene in Surti bulls. The observed SSCP variants having association with semen parameter shows the possibility of using AQP7 gene as a candidate gene for identification of markers for semen quality traits in buffaloes.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/380883
คำสำคัญ (EN): India
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Genetic polymorphism of aquaporin 7 gene and its association with semen quality in Surti bulls (Buffalo Bulletin (Thailand),37(2))
Beena Sinha
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Screening for genetic disorders in Indian Murrah and Surti buffalo (Bubalus bubalis) bulls (Buffalo Bulletin (Thailand),36(1)) Effect of feed energy levels on semen quality and freezability of young Murrah buffalo bulls (Buffalo Bulletin (Thailand),36(2)) Effect of nongenetic factors on semen production charecteristics of Murrah buffalo bulls at organized semen station (Buffalo Bulletin (Thailand),36(1)) Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull (Buffalo Bulletin (Thailand),35(1)) Studies on seminal attributes in relation to chromosomal profile in Murrah buffalo bulls (Buffalo Bulletin (Thailand),37(1)) Infectious bovine keratoconjunctivitis in a buffalo calf (Buffalo Bulletin (Thailand),37(1)) TLR-4 gene polymorphism in Indian Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin,37(1)) DGAT1 gene polymorphisms and its association with milk production traits in Mehsana buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(2)) Quality characteristics of fermented buffalo beef sausage (Buffalo Bulletin (Thailand),35(4)) Prevalence of cryptosporidiosis in buffalo calves of Jabalpur, India (Buffalo Bulletin (Thailand),37(1))
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