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A natural Vibrio parahaemolyticus ΔpirA Vp pirB Vp+ mutant kills shrimp but produces neither Pir Vp toxins nor acute hepatopancreatic necrosis disease lesions
Phiwsaiya K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A natural Vibrio parahaemolyticus ΔpirA Vp pirB Vp+ mutant kills shrimp but produces neither Pir Vp toxins nor acute hepatopancreatic necrosis disease lesions
ผู้แต่ง / หัวหน้าโครงการ (EN): Phiwsaiya K.
บทคัดย่อ (EN): Acute hepatopancreatic necrosis disease (AHPND) of shrimp is caused by Vibrio parahaemolyticus isolates (VP AHPND isolates) that harbor a pVA plasmid encoding toxins PirA Vp and PirB Vp . These are released from V PAHPND isolates that colonize the shrimp stomach and produce pathognomonic AHPND lesions (massive sloughing of hepatopancreatic tubule epithelial cells). PCR results indicated that V. parahaemolyticus isolate XN87 lacked pirA Vp but carried pirB Vp . Unexpectedly, Western blot analysis of proteins from the culture broth of XN87 revealed the absence of both toxins, and the lack of PirB Vp was further confirmed by enzyme-linked immunosorbent assay. However, shrimp immersion challenge with XN87 resulted in 47% mortality without AHPND lesions. Instead, lesions consisted of collapsed hepatopancreatic tubule epithelia. In contrast, control shrimp challenged with typical VP AHPND isolate 5HP gave 90% mortality, accompanied by AHPND lesions. Sequence analysis revealed that the pVA plasmid of XN87 contained a mutated pirA Vp gene interrupted by the out-of-frame insertion of a transposon gene fragment. The upstream region and the beginning of the original pirA Vp gene remained intact, but the insertion caused a 2-base reading frameshift in the remainder of the pirA Vp gene sequence and in the downstream pirB Vp gene sequence. Reverse transcription-PCR and sequencing of 5HP revealed a bicistronic pirAB Vp mRNA transcript that was not produced by XN87, explaining the absence of both toxins in its culture broth. However, the virulence of XN87 revealed that some V. parahaemolyticus isolates carrying mutant pVA plasmids that produce no Pir Vp toxins can cause mortality in shrimp in ponds experiencing an outbreak of early mortality syndrome (EMS) but may not have been previously recognized to be AHPND related because they did not cause pathognomonic AHPND lesions. © 2017 American Society for Microbiology.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026555961&doi=10.1128%2fAEM.00680-17&partnerID=40&md5=83b7ab0784042dd99562fad8335eef92
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Vibrio parahaemolyticus
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Phiwsaiya K.. "A natural Vibrio parahaemolyticus ΔpirA Vp pirB Vp+ mutant kills shrimp but produces neither Pir Vp toxins nor acute hepatopancreatic necrosis disease lesions". ไม่ระบุหน่วยงาน. ม.ป.ป.

Phiwsaiya K.. "A natural Vibrio parahaemolyticus ΔpirA Vp pirB Vp+ mutant kills shrimp but produces neither Pir Vp toxins nor acute hepatopancreatic necrosis disease lesions". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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A natural Vibrio parahaemolyticus ΔpirA Vp pirB Vp+ mutant kills shrimp but produces neither Pir Vp toxins nor acute hepatopancreatic necrosis disease lesions
Phiwsaiya K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Update on early mortality syndrome/acute hepatopancreatic necrosis disease by April 2018 Characterization and PCR detection of binary, pir-like toxins from vibrio parahaemolyticus isolates that cause acute hepatopancreatic necrosis disease (AHPND) in shrimp Development of monoclonal antibodies specific to ToxA and ToxB of Vibrio parahaemolyticus that cause acute hepatopancreatic necrosis disease (AHPND) Protein extract from red seaweed Gracilaria fisheri prevents acute hepatopancreatic necrosis disease (AHPND) infection in shrimp PirA & B toxins discovered in archived shrimp pathogenic Vibrio campbellii isolated long before EMS/AHPND outbreaks Vibrio biofilm inhibitors screened from marine fungi protect shrimp against acute hepatopancreatic necrosis disease (AHPND) Plasmid dynamics in Vibrio parahaemolyticus strains related to shrimp Acute Hepatopancreatic Necrosis Syndrome (AHPNS) Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND) Ginger and its component shogaol inhibit Vibrio biofilm formation in vitro and orally protect shrimp against acute hepatopancreatic necrosis disease (AHPND) Facts, truths and myths about SPF shrimp in Aquaculture
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