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DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand
Sumruayphol S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Sumruayphol S.
บทคัดย่อ (EN): Aedes aegypti (Diptera: Culicidae) (L.), Ae. albopictus (Skuse), and Ae. scutellaris (Walker) are important mosquito vectors of dengue and chikungunya viruses. They are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. Any confusion could have a negative impact on epidemiological studies or control strategies. Therefore, we explored two modern tools to supplement current morphological identification: DNA barcoding and geometric morphometric analyses. Field larvae were reared to adults and carefully classified based on morphological traits. The genetic analysis was based on the 658 bp each of 30COI sequences. Some Culex spp., Mansonia bonneae, were included as outgroups, and inclusion of a few other Aedes spp. facilitated phylogenetic inference of the relationship between Ae. albopictus and Ae. scutellaris. The two species were separated by an average interspecific divergence of 0.123 (0.119-0.127). Morphometric examination included landmark- (392 specimens) and outline-based (317 specimens) techniques. The shape of the wing showed different discriminating power based on sex and digitizing technique. This is the first time that Ae. scutellaris and Ae. albopictus have been compared using these two techniques. We confirm that these morphologically close species are valid, and that geometric morphometrics can considerably increase the reliability of morphological identification. © 2016 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961844226&doi=10.1016%2fj.actatropica.2016.03.010&partnerID=40&md5=4c60c27d50204c01e1ba6c186e3ac172
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Wings, Animal
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand
Sumruayphol S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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