สืบค้นงานวิจัย
A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs)
Prè D. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs)
ผู้แต่ง / หัวหน้าโครงการ (EN): Prè D.
บทคัดย่อ (EN): Many protocols have been designed to differentiate human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) into neurons. Despite the relevance of electrophysiological properties for proper neuronal function, little is known about the evolution over time of important neuronal electrophysiological parameters in iPSC-derived neurons. Yet, understanding the development of basic electrophysiological characteristics of iPSC-derived neurons is critical for evaluating their usefulness in basic and translational research. Therefore, we analyzed the basic electrophysiological parameters of forebrain neurons differentiated from human iPSCs, from day 31 to day 55 after the initiation of neuronal differentiation. We assayed the developmental progression of various properties, including resting membrane potential, action potential, sodium and potassium channel currents, somatic calcium transients and synaptic activity. During the maturation of iPSC-derived neurons, the resting membrane potential became more negative, the expression of voltage-gated sodium channels increased, the membrane became capable of generating action potentials following adequate depolarization and, at day 48-55, 50% of the cells were capable of firing action potentials in response to a prolonged depolarizing current step, of which 30% produced multiple action potentials. The percentage of cells exhibiting miniature excitatory post-synaptic currents increased over time with a significant increase in their frequency and amplitude. These changes were associated with an increase of Ca2+ transient frequency. Co-culturing iPSC-derived neurons with mouse glial cells enhanced the development of electrophysiological parameters as compared to pure iPSC-derived neuronal cultures. This study demonstrates the importance of properly evaluating the electrophysiological status of the newly generated neurons when using stem cell technology, as electrophysiological properties of iPSC-derived neurons mature over time. © 2014 Prè et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904976176&doi=10.1371%2fjournal.pone.0103418&partnerID=40&md5=8873f3255515b2e6ab00847c95f6c184
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Time Factors
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Prè D.. "A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs)". ไม่ระบุหน่วยงาน. ม.ป.ป.

Prè D.. "A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs)". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs)
Prè D.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Generation of porcine induced-pluripotent stem cells from Sertoli cells Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation In vitro production of functional immune cells derived from human hematopoietic stem cells Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Enhancement of TNF-α induced antiproliferation in human cervical cancer cells by extracts from Thai medicinal plants Antisense oligonucleotide induction of progerin in human myogenic cells Biology of Zika virus infection in human skin cells Domesticated animals and human infectious diseases of zoonotic origins: Domestication time matters Full genomic analysis of a human G9P[23] rotavirus strain from a child with diarrhea in Thailand
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พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
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