สืบค้นงานวิจัย
Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection
Kerdmusik C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection
ผู้แต่ง / หัวหน้าโครงการ (EN): Kerdmusik C.
บทคัดย่อ (EN): The hepatopancreas of the shrimp is the target organ of several pathogens. Diagnosis of diseases of the hepatopancreas usually required an isolation of the organ and the process cannot avoid sacrificing the shrimp. To save life of the animal, this study was aimed at taking hepatopancreatic tissue from the shrimp by biopsy process. Thirty-two sexually mature adults of the black tiger shrimp Penaeus monodon were used as the shrimp model and small pieces of their hepatopancreatic tissue were taken by needle biopsy. Specific position of the needle penetration was suggested to avoid damage to the heart and the gills. From the lateral view of the carapace of the shrimp, this position was at one-third from the rostral end of a line drawn from the last dorsal spine of the rostrum to the posterior margin of the carapace. From the top view, this point was located at a medial quarter of a line drawn from the midline of the carapace toward the lateral extremity. The needle was inserted vertically at a depth of 1 cm into the hepatopancreas, and small pieces of the tissue were quickly withdrawn into the syringe. After the operation, the shrimp were released into rearing tanks and observed for any mortality or morbidity for two months, during which all the shrimp survived and remained apparently healthy. The hepatopancreatic tissues taken from the biopsy were processed for polymerase chain (PCR) reaction method, fresh smear and histology. The PCR revealed the presence of Penaeus monodon densovirus (PmoDNV) in 40% of the samples. The fresh smear and histology revealed good quality of cell morphology and histological features, as well as PmoDNV inclusions in the PCR-positive samples. This study suggests that needle biopsy of hepatopancreas to detect pathogens, or to evaluate health status, of this organ in shrimp is possible, at least in P. monodon. © 2018 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042209247&doi=10.1016%2fj.aquaculture.2018.02.019&partnerID=40&md5=9e12fb74503c975372486941df50cf0d
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Penaeus monodon
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Kerdmusik C.. "Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kerdmusik C.. "Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection
Kerdmusik C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon A novel function of bursicon in stimulation of vitellogenin expression in black tiger shrimp, Penaeus monodon A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon Optimisation of electroporation and lipofection protocols to derive the black tiger shrimp cell line (Penaeus monodon) An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon) Evidence that the whiteleg shrimp Penaeus (Litopenaeus) vannamei is refractory to infection by Penaeus monodon nudivirus (PmNV), also known as MBV (ภาษาอังกฤษ) Functional characterization of a novel C-type lectin gene in the black tiger shrimp, Penaeus monodon
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