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Investigation of colchicine incubation time on the regeneration rate of Globba williamsiana ‘Dok Khao’
Chuengpanya R. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Investigation of colchicine incubation time on the regeneration rate of Globba williamsiana ‘Dok Khao’
ผู้แต่ง / หัวหน้าโครงการ (EN): Chuengpanya R.
บทคัดย่อ (EN): Globba williamsiana ‘Dok Khao’ is a native ginger of Thailand. This plant has the outstanding characteristics of bract and inflorescence which have been used as an ornamental ginger for cut-flowers and potted plants. To increase the economic value of this plant, improvement is required. Chromosome doubling by colchicine is a general method for improving most plant species. Therefore, this study aimed to investigate the effect of colchicine incubation time on the regeneration rate of G. williamsiana ‘Dok Khao’ before establishing the effective protocol for producing tetraploid plants. Changing in morphological and anatomical characters of M1V1 plants were also observed. After incubating young leafy-shoots, 1.5 cm high, with 0.2% (w/v) colchicine for 0, 4, 8 and 12 days, all explants were transferred to MS gelrite medium for 8 weeks. The results showed that survival rate was decreased while incubation time increased and calculated LT50 was 6.9 days. The regeneration rate in term of M1V1 leafy-shoot number, leafy-shoot height, root number, and root length were observed. Three from four parameters of regeneration rate, except leafy-shoot numbers of M1V1, was significantly reduced after longer exposed with colchicine. The development of leafy-shoot and leaf morphology of M1V1 plants were also affected by colchicine. Changing in anatomy of some M1V1 plants, such as enlargement of stomata and epidermal cell size, reduction of epidermal cell density per unit area, and disorganization of epidermal cell were observed. These morphological and anatomical characteristics may benefit for primary screening the tetraploids from diploids. © 2017, International Society for Horticultural Science. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028938410&doi=10.17660%2fActaHortic.2017.1167.23&partnerID=40&md5=c555cee2c2167d06baf55aa6e216ef2e
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Zingiberaceae
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Investigation of colchicine incubation time on the regeneration rate of Globba williamsiana ‘Dok Khao’
Chuengpanya R.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Effect of colchicine incubation time on tetraploid induction of Kaempferia rotunda อิทธิพลของสาร Colchicine ต่อส่วนต่างๆ ของหม่อนที่เลี้ยงในอาหารเลี้ยงเนื้อเยื่อ Dependence of the neutron monitor count rate and time delay distribution on the rigidity spectrum of primary cosmic rays Effects of hydraulic retention time and organic loading rate on performance and stability of anaerobic digestion of Spirulina platensis Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand The development of resistivity equipment for subsoil investigation Investigation of Transcription Factor bHLH in Hevea การใช้ Time Domain Reflectometry (TDR) ในการวัดปริมาณความชื้นในดิน Measuring Soil Moisture Content with Time Domain Reflectometry Morbidity Rate Prediction of Dengue Hemorrhagic Fever (DHF) Using the Support Vector Machine and the Aedes aegypti Infection Rate in Similar Climates and Geographical Areas Follicular growth, time of ovulation and conception rate after synchronization with medroxy progesterone acetate impregnated sponges in Nili Ravi buffalo heifers (Buffalo Bulletin (Thailand),36(2))
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