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Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa
Buranaamnuay K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa
ผู้แต่ง / หัวหน้าโครงการ (EN): Buranaamnuay K.
บทคัดย่อ (EN): Effects of Equex and glycerol additions and sample dilution step on frozen-thawed epididymal cat spermatozoa were investigated. The epididymal sperm pellets were resuspended in extenders using one- (groups III and IV) or two- (groups I, II, V and VI) step dilution. For one-step dilution, the pellets were resuspended in plain egg yolk-Tris medium (EYT) + 5% glycerol with (IV)/without (III) 0.5% Equex and cooled (4°C, 1 h). For two-step dilution, the pellets were resuspended in EYT (I and V) and in EYT + 3% glycerol (II and VI), cooled and further diluted with EYT + 10% glycerol with (I)/without (V) 1% Equex and with EYT + 7% glycerol with (II)/without (VI) 1% Equex. Immediately after freeze-thawing, no differences (p > 0.05) were found in the motility, viability and membrane integrity (HOST) among the groups except the lowest HOST in IV (p = 0.005 to p = 0.04). The acrosome integrity (FITC) in group I was comparable to that in group II (p > 0.05) and was higher than the rest (p < 0.001 to p = 0.02). At 2 h after thawing, the motility, viability and HOST were comparable among the groups (p > 0.05) except the lower percentages of viability in III (p = 0.008 to p = 0.3) and of HOST in IV (p = 0.005 to p = 0.2). Two-step dilutions with Equex (I, II) were more beneficial for the FITC at 2 h than without Equex (V) (p = 0.005 and p = 0.02) and than one-step dilutions (III, IV) (p < 0.001 to p = 0.02). In conclusion, epididymal cat sperm quality after freeze-thawing could be improved when Equex was added and two-step dilution was performed during freezing. The extenders prepared for the first step of dilution could be with (3%) or without (0%) glycerol. © 2015 Blackwell Verlag GmbH.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928266645&doi=10.1111%2frda.12496&partnerID=40&md5=3a379dd5ecb37a0c7c09f2daea4b5257
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Spermatozoa
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa
Buranaamnuay K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
การเก็บรักษาพันธุ์กล้วยไม้ระยะยาวในพื้นที่โคกภูตากาโดยวิธี cryopreservation Protocols for sperm cryopreservation in the domestic cat: A review Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility Vitrification-based cryopreservation of Dendrobium cruentum Rchb. F. Seeds Effect of extracellular adenosine 5'-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability Low-density lipoprotein improves motility and plasma membrane integrity of cryopreserved canine epididymal spermatozoa Cryopreservation technique of chilli seeds by vitrification Cryopreservation induces capacitation-like changes of the swamp buffalo spermatozoa (Buffalo Bulletin (Thailand),36(1)) Dehydration effects on cryopreservation of Cymbidium finlaysonianum protocorms
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