คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Tomohide

เผยแพร่โดย

1 Department of Virology and Parasitology, Fujita Health University, School of Medicine Aichi, Japan 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 4 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Whole genomic analysis of bovine group A rotavirus strains A5-10 and A5-13 provides evidence for close evolutionary relationship with human rotaviruses Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand: Evidence for independent porcine-to-human interspecies transmission events Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Ultra Structure changes of diosgenin-treated human monocyte U937-derived macrophages induced by naegleria fowleri lysate ฤทธิ์ทางชีวภาพต่อเซลล์ human skin fibroblast และ B16F10 melanoma ของสารสกัด รวงข้าวขาวดอกมะลิ 105 ฤทธิ์ทางชีวภาพต่อเซลล์ human skin fibroblast และ B16F10 melanoma ของสารสกัด รวงข้าวขาวดอกมะลิ 10 Emergence and Characterization of Unusual DS-1-Like G1P[8] Rotavirus Strains in Children with Diarrhea in Thailand Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand

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ชื่อเรื่อง (EN):	Full genomic analysis of a human G9P[23] rotavirus strain from a child with diarrhea in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Tomohide
บทคัดย่อ (EN):	bjective: Pigs are considered to be a major potential reservoir for emerging RVAs affection humans. P[23] is a relatively rare P genotype detected only in pigs so far in combination with G2, G3, G4, G5, or G9 genotype. P[23] was first identified in a Venezuelan strain, RVA/Pig-tc/VEN/A34/1985/G5P[23]. And subsequently in Brazil, Asia and Europe. Among the collected stool samples in Thailand in 2014, We isolated a human G9P[23] strain. KKL-117. From a diarrheic child. To elucidate the origin and evaluation of this strain, we analyzed the whole genome. Materials and Methods: The whole genome of strain KKL-117 were amplified using a sequence-independent primer set and then sequenced using the next generation sequencing Illumina MiSeq plat form. Data analysis was performed using CLC Genomic Workbench v8.0.1. Contigs were assembled from the obtained sequence reads by de novo assembly. Using the assembled contigs as query sequences, the BLAST non-redundant nucleotide database was searched to obtain the full length nucleotide sequence of each gene segment of strains KKL-117. Phylogenic trees were constructed using the maximum likelihood method using MEGA6.06. Result and discussion: On whole genomic analysis, the 11 genes of strain KKL-117 were assigned as G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Strain KKL-117 exhibited a unique non G/P genotype constellation (I5-R1-C1-M1-A8-N1-T1-E1H1). Which is commonly found in RVA strains form pigs. Our findings add to the increasing evidence supporting animal to-human interspecies transmission. The porcine origin of strain KKL-117 suggests interspecies transmission due to the close proximity between humans and livestock, especially in developing countries in Asia where there is intimate contact between humans and livestock such as pigs. Continuing RVA surveillance of this G9P[23] genotype in humans is required.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=645
เผยแพร่โดย (EN):	1 Department of Virology and Parasitology, Fujita Health University, School of Medicine Aichi, Japan 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 4 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand
คำสำคัญ (EN):	human
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science