สืบค้นงานวิจัย
Successful derivation of xeno-free mesenchymal stem cell lines from endometrium of infertile women
Phermthai T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Successful derivation of xeno-free mesenchymal stem cell lines from endometrium of infertile women
ผู้แต่ง / หัวหน้าโครงการ (EN): Phermthai T.
บทคัดย่อ (EN): Transplantation of mesenchymal stem cells (MSC) can effectively repair endometrial deficiencies, including infertile patients with a problem of inadequate endometrium thickness. Although, MSC derived from different organ sources have a similarity of MSC specific characteristics, endometrial stem cells (EMSC) are temporally regulated throughout the menstrual cycle in a micro-environmental niche found only in endometrial tissue. Given the micro-environment niche, developing treatments for endometrial disorders with EMSC should be a top priority. To provide EMSC that afford safety for therapeutic usage, we have established a completely xeno-free EMSC line derivation protocol using human allogenic umbilical cord serum instead of animal derived reagents, and proved that it is feasible to generate xeno-free EMSC lines from infertile patient donors using these conditions. Our results demonstrate the successful derivation of xeno-free EMSC lines from 10 out of 10 infertile patients. The resultant xeno-free EMSC lines showed typical MSC morphology, phenotypic markers, differentiation capacity, telomere length and normal karyotypes. They showed superior proliferation capability, but lower expression of proto-oncogenes, to the lines generated under standard (animal derived reagents) culture. Biosafety of xeno-free EMSC lines also displayed in retention of immunosuppressive ability, epigenetic stability by imprinted genes expression, proto-oncogenes expression and no mutation of specific codon on p53 tumor suppressor gene. Taken together, these data indicate that our cells may be safe for clinical use. In conclusion, we have succeeded in establishing completely xeno-free EMSC lines and demonstrate for the first time that autogenic and xeno-free EMSC lines can be generated from infertile women. © 2016 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84997173330&doi=10.1016%2fj.repbio.2016.10.002&partnerID=40&md5=1cbf42821960317a8c74da992d3e13bb
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Mesenchymal Stromal Cells
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Phermthai T.. "Successful derivation of xeno-free mesenchymal stem cell lines from endometrium of infertile women". ไม่ระบุหน่วยงาน. ม.ป.ป.

Phermthai T.. "Successful derivation of xeno-free mesenchymal stem cell lines from endometrium of infertile women". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Successful derivation of xeno-free mesenchymal stem cell lines from endometrium of infertile women
Phermthai T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
A genetically immortalized human stem cell line: A promising new tool for Alzheimer’s disease therapy Genomic alteration in Head and Neck Squamous Cell Carcinoma (HNSCC) cell lines inferred from karyotyping, molecular cytogenetics, and array comparative genomic hybridization Computational identification of miRNAs that modulate the differentiation of mesenchymal stem cells to osteoblasts Real time monitoring cell proliferation of SW620 colorectal cancer cell line following co-culture with PHA-stimulated lymphocytes and CD44 expression following cytotoxic and apoptotic induction RNAi screen reveals a role of SPHK2 in dengue virus–mediated apoptosis in hepatic cell lines Notch-1 Signaling Mediated Dendritic cells - Induced Autologous NK Cell Proliferation and Cytotoxicity Activity to Cancer Cell In vitro generation of dendritic cell - breast cancer cell hybrids for induction of cellular immunity แนวทางการควบคุมคุณภาพผลิตภัณฑ์เซลล์ต้นกำเนิดสำเร็จรูป(stem cell products)ทางห้องปฏิบัติการ Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation Characteristics and potential utilizations of starch from pineapple stem waste
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