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Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle (Buffalo Bulletin,41(2))
Parthiban Manoharan - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle (Buffalo Bulletin,41(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Parthiban Manoharan
หน่วยงานสังกัดผู้แต่ง (EN): Chennai (India). Department of Animal Biotechnology
บทคัดย่อ (EN): The aim of the present study was to identify the gene expression profile of three candidate genes namely heat shock protein 70, oviductal glycoprotein and osteopontin in buffalo oviduct during different stages of the oestrous cycle. Slaughter house derived buffalo oviducts were categorized as belonging to early, mid and late phase of the oestrous cycle based on the morphological appearance of CL and progesterone concentration. The relative abundance of each of the candidate gene transcript was quantified by real time RT-PCR. Transcripts of all the three candidate genes were found to be expressed in OEC throughout the oestrous cycle. The results of the present study demonstrate that gene expression in the buffalo oviduct is clearly regulated during the oestrous cycle, as these candidate genes play a crucial role in several reproductive processes. It was found that Hsp 70 gene was significantly upregulated in OEC belonging to the early and late phases when compared to mid phases of the cycle. The expression of OVGP mRNA was found to be maximum during the early phase and it remained low during the mid and late phases while expression of OPN mRNA was maximum during the early phase and declined sequentially during the mid and late phase of oestrus cycle.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/418063
คำสำคัญ (EN): Quantitative PCR
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Expression profiling of candidate embryotrophic genes of buffalo oviduct during different stages of oestrous cycle (Buffalo Bulletin,41(2))
Parthiban Manoharan
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Expression and structural variations of toll-like receptor 4 gene in buffalo and cattle (Buffalo Bulletin,41(1)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Effect of different rearing systems on behavioural responses to novel stimuli in Murrah buffalo calves (Buffalo Bulletin,41(4)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Constraints of buffalo farming in Kallakurichi district of Tamil Nadu, India (Buffalo Bulletin,41(3)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1)) Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine (Buffalo Bulletin,41(3)) Clinical management of hydramnios coupled with bull dog calf in a Jaffarabadi buffalo (Buffalo Bulletin,41(4))
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