สืบค้นงานวิจัย
Functional Genomics and Bioinformatics-Assisted Gene Marker Development for Economically Important Traits in Rubber
Emma K. Sales - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Functional Genomics and Bioinformatics-Assisted Gene Marker Development for Economically Important Traits in Rubber
ผู้แต่ง / หัวหน้าโครงการ (EN): Emma K. Sales
บทคัดย่อ (EN): The increasing demand of natural rubber products by the automotive, industrial, construction and medical supplies industries has renewed interest in revitalizing rubber production in the Philippines. This can be achieved by increasing the hectarage planted to rubber and the use of clones with improved traits such as high yield and pest and disease resistance. Towards attaining such goals, research was carried out in the areas of functional genomics and bioinformatics to develop gene markers for important agronomic traits. A study was undertaken to (a) develop and establish gene markers and ESTs for a library database in Hevea brasiliensis, (b) sequence gene specific regions in rubber using NGS technology, (c) construct an exome database for at least two H. brasiliensis genotypes with extreme phenotypic traits, (d) search for genespecific markers from the exome database and (e) design PCR primers for gene-specific sequences and validate these primers for trait association. Seven NSIC recommended varieties (PB260, RRIM600, PB330, PB217, PB311, USM1, and PR107) were selected as test samples. Genomic DNAs of these samples were isolated for library preparation using rare cutter restriction enzymes for subsequent cloning. A total of 133 colonies were sequenced and subjected to BLAST and gene ontology analysis. DNA sequences were obtained that bore similarities to genes involved in 18 biological processes, 8 cellular components and 9 molecular functions. SSR primers were developed and selected for preliminary testing and utilization of differential banding patterns among the seven NSIC recommended clones.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): SSR
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Functional Genomics and Bioinformatics-Assisted Gene Marker Development for Economically Important Traits in Rubber
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