สืบค้นงานวิจัย
Effects of Addition of Reduced Glutathione to Thawing media on Motility Parameters, Lipid Peroxidation and Fertility Rate in Frozen-Thawed Chicken Spermatozoa
K. Sakwiwatkul - มหาวิทยาลัยขอนแก่น
ชื่อเรื่อง (EN): Effects of Addition of Reduced Glutathione to Thawing media on Motility Parameters, Lipid Peroxidation and Fertility Rate in Frozen-Thawed Chicken Spermatozoa
ผู้แต่ง / หัวหน้าโครงการ (EN): K. Sakwiwatkul
หน่วยงานสังกัดผู้แต่ง:
บทคัดย่อ (EN): Sperm cryopreservation represents a useful tool in genetic conservation management of indigenous in chicken. However, freezing and thawing produce physical and chemical stress on the sperm membrane that reduces their viability and fertilizingability. The objective of the present study was, therefore, to evaluated the effects of reduced glutathione (GSH; 0.1, 0.5 and 1 mM) supplementation in the thawing diluent on post-thawed parameters of frozen chicken spermatozoa. Semen was collected from 24 Thai native chickens (Pradu Hang Dum). Semen sample were frozenby liquid nitrogen vapor method. After thawing, diluent supplementation with each of GSH concentrations. Sperm motility characteristics were assayed by CASA, ROS generation was assessed by TBARSand fertility test of frozen semen was carried on by inseminated to layer hens. The results showed that addition of 0.1, 0.5 and 1 mM GSH to the thawing media decreased the percentage of motile, progressively motile spermatozoa and fertility rate. Whereas, addition of GSH to the thawing media resulted in a reduction in ROS generation.Our results do not support the use of GSH (0.1 to 1 mM) to improve the quality of post-thawed chicken semen.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
จำนวนหน้า: 5
เอกสารแนบ: https://ag2.kku.ac.th/kaj/PDF.cfm?filename=O17 983_P. Thananurak.pdf&id=2004&keeptrack=1
เผยแพร่โดย: มหาวิทยาลัยขอนแก่น
คำสำคัญ (EN): native fowl
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Effects of Addition of Reduced Glutathione to Thawing media on Motility Parameters, Lipid Peroxidation and Fertility Rate in Frozen-Thawed Chicken Spermatozoa
K. Sakwiwatkul
มหาวิทยาลัยขอนแก่น
2558
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