สืบค้นงานวิจัย
Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor
Chutichetpong P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor
ผู้แต่ง / หัวหน้าโครงการ (EN): Chutichetpong P.
บทคัดย่อ (EN): Drug susceptibility testing (DST) for Mycobacterium tuberculosis currently faces multiple challenges, including lengthy ineffective standard methods, the expensive cost of molecular tests, and large bulky diagnostic machines. In this work, a disposable MPT64 sensor was developed to rapidly determine drug susceptibility (DS-TB) and multidrug resistant tuberculosis (MDR-TB) using an electrochemical sandwich-immunosensor for the detection of MPT64 as an indicator of Mycobacterium tuberculosis growth. Anti-MPT64 (as capture antibody; cAb) was immobilised on screen printed carbon electrodes to specifically bind with MPT64 target protein. A reporter probe of anti-MPT64 conjugated with horseradish peroxidase (HRP labeled rAb) then completed the sandwich immunocomplex. The current signals were received from the catalytic reaction between 3,3′-5,5′-tetramethylbenzidine (TMB), H 2 O 2 and HRP labeled rAb using the chronoamperometric mode on a portable potentiostat. Increasing MPT64 concentration was taken as an indicator of growth, which was measured by the disposable MPT64 sensor. This sensor shows the possibility of determining DST by comparing the signals of DS-TB and MDR-TB growth in drug-free and drug-containing liquid medium. The time required to identify DS-TB and MDR-TB in pure culture MTB and leftover sputum sediments from patients are 3 days and 4–6 days, respectively. Therefore, using this sensor is significantly rapid and inexpensive and has the potential to be used for drug susceptibility testing of tuberculosis in middle to low income countries. © 2018 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046832277&doi=10.1016%2fj.aca.2018.04.064&partnerID=40&md5=035b276499deb43581d892ee51b52c41
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tuberculosis, Multidrug-Resistant
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Chutichetpong P.. "Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chutichetpong P.. "Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor
Chutichetpong P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Rapid screening for anthocyanins in cane sugars using ESR spectroscopy Evaluation of HBsAg rapid test kits in Thailand A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis Tuberculin skin test and QuantiFERON-TB Gold In-tube Test for latent tuberculosis in Thai HIV-infected adults Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand. Whole-genome sequencing analysis of serially isolated multi-drug and extensively drug resistant Mycobacterium tuberculosis from Thai patients Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test Comparing interferon-gamma release assays to tuberculin skin test in thai children with tuberculosis exposure Tuberculin skin test and QuantiFERON®-TB Gold In-tube Test for diagnosing latent tuberculosis infection among Thai healthcare workers
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