สืบค้นงานวิจัย
A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis
Saengdee P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis
ผู้แต่ง / หัวหน้าโครงการ (EN): Saengdee P.
บทคัดย่อ (EN): A silicon nitride Ion Sensitive Field Effect Transistor (ISFET) based immunosensor was developed as a low-cost and label-free electrical detection for the detection of antigen 85 complex B (Ag85B). The sensing membrane of the ISFET was modified with 3-aminopropyltriethoxysilane (APTES) followed by glutaraldehyde (GA), yielding an aldehyde-terminated surface. This group is available for immobilization of a monoclonal antibody against a recombinant Ag85B protein (anti-Ag85B antibody). The optimal concentration for anti-Ag85B antibody immobilization onto the modified ISFET was 100 μg ml-1. This optimal condition provided the maximal binding capability and minimal non-specific background signal. The binding event between the recombinant Ag85B antigen and anti-Ag85B antibody on the ISFET surface is presented by monitoring the gate potential change at a constant drain current. The dose response for the recombinant Ag85B protein showed a linear response between 0.12 and 1 μg ml-1 without significant interference from other recombinant proteins. The analytical imprecision (CV%) and accuracy of this Ag85B protein biosensor were 9.73-10.99% and 95.29%, respectively. In addition, an irrelevant antibody and other recombinant proteins were employed as a negative control to demonstrate the non-specific interaction of the antigen and antibody. The success of this immunosensor system for Ag85B protein detection facilitates the construction of a promising device which can shorten the turnaround time for the diagnosis of tuberculosis compared to a standard culture method. Furthermore, this device could also be applied for real-time growth monitoring of Mycobacterium tuberculosis in a mycobacterial culture system. © 2016 The Royal Society of Chemistry.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990061917&doi=10.1039%2fc6an00568c&partnerID=40&md5=ed238e48d1d67156c4ae60f526baaa26
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tuberculosis
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Saengdee P.. "A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Saengdee P.. "A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis
Saengdee P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test Rapid molecular detection of multidrug- resistant tuberculosis by PCR-nucleic acid lateral flow immunoassay Rapid detection of tuberculosis resistant to isoniazid and rifampicin by real-time PCR and melting curve analysis The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture Delay in treatment initiation and treatment outcomes among adult patients with multidrug-resistant tuberculosis at Yangon Regional Tuberculosis Centre, Myanmar: A retrospective study Development of molecular approach based on PCR assay for detection of histamine producing bacteria Pesticide analysis using nanoceria-coated paper-based devices as a detection platform Rapid screening drug susceptibility test in tuberculosis using sandwich electrochemical immunosensor Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of Paramphistomum gracile circulating 16 kDa antigen
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair