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Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. Carrying the gibberellin 2-oxidase gene
Otani M. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. Carrying the gibberellin 2-oxidase gene
ผู้แต่ง / หัวหน้าโครงการ (EN): Otani M.
บทคัดย่อ (EN): The gibberellin 2-oxidase catalyzes the bioactive gibberellins or their immediate precursors to inactive forms. We have previously produced transgenic plants of the liliaceous plant Tricyrtis sp. containing the GA2ox gene from the linderniaceous plant Torenia fournieri. These transgenic plants showed dwarf phenotypes as expected but unfortunately had no flowers or only small, unopened flowers. Recently, one newly produced transgenic line (G2-55) formed fully opened flowers. G2-55 showed a moderately dwarf phenotype and the shoot length decreased to 63.4% of that of the control, non-transgenic plants. No significant differences in the number of flowers per shoot and in the flower size were observed between G2-55 and the control. Flow cytometry analysis and chromosome observation showed that G2-55 was tetraploid (2n=4x=52), whereas the other transgenic lines producing no or only small flowers were diploid (2n=2x=26) as the mother plant. Pollen fertility of G2-55 was 81.2% as determined by acetocarmine staining. The tetraploidy in G2-55 might be resulted from somaclonal variation of embryogenic calluses used as a target material for Agrobacterium-mediated transformation. The tetraploid transgenic plant G2-55 may be useable not only directly as a potted plant, but also as a material for further breeding of Tricyrtis spp. © 2014 The Japanese Society for Plant Cell and Molecular Biology.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940823038&doi=10.5511%2fplantbiotechnology.14.0916a&partnerID=40&md5=7dcda94b46c4dd320d5d0f9e0f4d8a3c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tricyrtis
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. Carrying the gibberellin 2-oxidase gene
Otani M.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis การส่งถ่ายยีน Antisense ACC Oxidase สู่โปรโตคอร์มกล้วยไม้เอื้องดอกมะขาม (Dendrobium delacourii Guill) โดยใช้ Agrobacterium tumefaciens เป็นตัวกลาง Genetic Transformation and Regeneration of Hevea Brasiliensis Transgenic Plant with GAI Gene Cloning of Arabidopsis WUS Gene, Construcion and Identification of WUS-EGFP Fusion Gene Plant Expression Vector Erratum to: Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua L. (Plant Cell, Tissue and Or Lamtakhong_Plant. Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii Characterization of the biosynthetic gene cluster for cryptic phthoxazolin a in streptomyces avermitilis Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii
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