สืบค้นงานวิจัย
Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis
Kuswanhadi - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Kuswanhadi
บทคัดย่อ (EN): Ethylene is a major stimulating factor for natural rubber production in Hevea brasiliensis and it is often applied in the form of Ethephon, an ethylene releaser. In a positive feedback type mechanism, the application of ethylene leads to the enhanced expression of genes involved in ethylene biosynthesis such as ACC oxidases, which are also responsive to wounding. We studied the regulation of ethylene biosynthesis in rubber tree through the characterization of the ACC oxidase multigene family and the responses of individual genes to ethylene and wounding. Three full-length cDNAs, HbACO1, HbACO2, HbACO3, were isolated. The HbACOs were 1115, 1174 and 1074 bp long respectively, with open reading frames encoding polypeptides of 312, 318 and 318 amino acids respectively. The genomic sequences of HbACO1 and HbACO2 were also characterised, revealing divergent gene structures: HbACO1 (1456 bp) possesses three introns and four exons, while HbACO2 (1418 bp) consists of two introns and three exons. All of the HbACO genes were expressed at all stages of development studied, from in vitro callus to the exploited plant, but with different expression profiles. HbACO1 was expressed at a higher level than the other genes, and was down-regulated by ethylene and wounding in both leaf and bark tissues. In contrast, HbACO2 and HbACO3 were transiently induced in response to ethylene and wounding. Treatment with 1-MCP, an ethylene action inhibitor, abolished the ethylene induction of HbACO2 expression, showing that this gene was under positive feedback regulation. The expression of HbACS3 was also induced by ethylene treatment and wounding, again with an antagonistic effect of 1-MCP. In conclusion, HbACO1 appears to be responsible for basal levels of ethylene production while HbACO2 and HbACO3 are up-regulated in response to external factors.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): latex cell
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Kuswanhadi. "Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kuswanhadi. "Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Effect of ethylene stimulation and wounding to the expression of three members of the multigene family encoding ACC oxidases in Hevea brasiliensis
Kuswanhadi
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Identification of a Multigene Family Encoding ACC Oxidase in Hevea brasiliensis Assessment of Low Frequency Tapping Systems with Stimulation on Hevea in Southern Thailand Production of Mutants Affected in Hormone Signalling to Dissect Defence Mechanisms in Hevea brasiliensis: The Case of Ethylene Effect of Three Rootstocks on the Same Scion PR107 of Hevea brasiliensis MicroRNAs: New Regulators of Biological Functions in Hevea brasiliensis Effect of Tapping Systems and Simulation on Clone RRIT 251 Effect of Panel Changing on Long Term Yield Response of Hevea brasiliensis (clone RRII 105) under Different Frequencies of Tapping and Stimulation Differential expression of some ASR gene isoforms in the latex and bark of rubber tree. Effects of Ethrel stimulation. Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis CLONAL VARIABILITY IN THE INCLINATION OF LATICIFERS AND PHLOIC RAYS IN HEVEA BRASILIENSIS
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair