สืบค้นงานวิจัย
Evaluation of agglutination strength by a flow-induced cell movement assay based surface plasmon resonance (SPR) technique
Sudprasert K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Evaluation of agglutination strength by a flow-induced cell movement assay based surface plasmon resonance (SPR) technique
ผู้แต่ง / หัวหน้าโครงการ (EN): Sudprasert K.
บทคัดย่อ (EN): A flow-induced cell movement assay combined with a surface plasmon resonance (SPR) technique was developed to quantify the agglutination strength, derived from the standard tube-agglutination test. Red blood cells (RBCs), based on the ABO blood group system, were specifically captured by anti-A and/or anti-B antibodies immobilized on a sensor surface. The agglutination strength corresponds to the amount of antigen-antibody interactions or the strength of RBC adhesion. Under a shear flow, the adherent RBCs were forced to move out of the region of interest with different average cell velocities (v c ) depending upon the adhesion strength and wall shear stress (WSS). That is, a higher adhesion strength (higher agglutination strength) or lower WSS represents a lower v c or vice versa. In this work, the agglutination strength was derived from the v c that was calculated from the time derivative of the relative SPR signal by using a simple model of cell movement response, whose validity was verified. The v c values of different samples were correlated with their agglutination strengths at a given WSS and antibody surface density. The v c decreased as the agglutination strength increased, which can be considered as a linear regression. The coefficient of variation of the calculated v c decreased to 0.1 as v c increased to 30 μm min -1 . The sensitivity of this assay can be controlled by optimizing the antibody surface density or the WSS. This assay has the capability to resolve the antigen density of A 1 and B RBCs from that of A 1 B RBCs. © The Royal Society of Chemistry 2015.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84921448103&doi=10.1039%2fc4an01779j&partnerID=40&md5=3eb864109ea5131fcfec347dac9a90c7
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Surface Plasmon Resonance
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Evaluation of agglutination strength by a flow-induced cell movement assay based surface plasmon resonance (SPR) technique
Sudprasert K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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