คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Dueantem Thongphueak

เผยแพร่โดย

1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. 2 Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok 10110, Thailand. 3 Innovative Learning Center, Srinakharinwirot University, Bangkok 10110, Thailand. 4 Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. 5 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand. 6 Center of Excellence in Biosensors, Srinakharinwirot University, Bangkok 10110, Thailand.

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสและเลปโตสไปโรสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis and leptosp Method verification of Isolated Chicken Eye Test Development of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for the detection of the causative agent of covid-19 การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital Development of a rapid, simple method for detecting Naegleria fowleri visually in water samples by Loop-Mediated Isothermal Amplification (LAMP) The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture Efficacy Evaluation Test 15min Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positiv การทดสอบผลผลิตและส่วนประกอบทางเคมีของหญ้าสกุล Brachiaria spp.ในพื้นที่จังหวัดมุกดาหาร

คัดลอก URL

ชื่อเรื่อง (EN):	Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs)
ผู้แต่ง / หัวหน้าโครงการ (EN):	Dueantem Thongphueak
บทคัดย่อ (EN):	The detection of Campylobacter spp. in meat products was developed by using loop-mediated isothermal amplification (LAMP) combined with DNA-based bioassay methods, including a lateral-flow dipstick (LFD) and gold nano-DNA probe (AuNPs) assay. The LAMP primers were designed from the conserved nucleotide regions of Campylobacter spp. The analytical sensitivity of the LAMP-LFD and LAMP-AuNPs analysis was 360 fg/μl. The analytical specificity of LAMP-based assays showed no cross-reactions to Listeria monocytogenes, Salmonella Typhimurium, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter aerogenes, Serratia marcescens, Vibrio parahaemolyticus, Vibrio cholerae, Klebsiella oxytoca and Citrobacter diversus. The sensitivity, specificity and accuracy of both LAMP-LFD and LAMP-AuNPs for the detection of pre-enrichment cultures from raw chicken meat samples were 100%, 95% and 96.67%, respectively. Since the processing time of LAMP-based assays is 60-90 minutes, it is applicable as a point-of-care screening test for food safety and as a process control of Campylobacter spp. contamination.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=548
เผยแพร่โดย (EN):	1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. 2 Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok 10110, Thailand. 3 Innovative Learning Center, Srinakharinwirot University, Bangkok 10110, Thailand. 4 Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. 5 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand. 6 Center of Excellence in Biosensors, Srinakharinwirot University, Bangkok 10110, Thailand.
คำสำคัญ (EN):	Campylobacter; Loop-mediated isothermal amplification; Lateral flow dipstick; Gold nano-DNA probe.
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science