สืบค้นงานวิจัย
Source-identifying biomarker ions between environmental and clinical Burkholderia pseudomallei using whole-cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
Niyompanich S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Source-identifying biomarker ions between environmental and clinical Burkholderia pseudomallei using whole-cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
ผู้แต่ง / หัวหน้าโครงการ (EN): Niyompanich S.
บทคัดย่อ (EN): Burkholderia pseudomallei is the causative agent of melioidosis, which is an endemic disease in Northeast Thailand and Northern Australia. Environmental reservoirs, including wet soils and muddy water, serve as the major sources for contributing bacterial infection to both humans and animals. The whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has recently been applied as a rapid, accurate, and high-throughput tool for clinical diagnosis and microbiological research. In this present study, we employed a whole-cell MALDI-TOF MS approach for assessing its potency in clustering a total of 11 different B. pseudomallei isolates (consisting of 5 environmental and 6 clinical isolates) with respect to their origins and to further investigate the source-identifying biomarker ions belonging to each bacterial group. The cluster analysis demonstrated that six out of eleven isolates were grouped correctly to their sources. Our results revealed a total of ten source-identifying biomarker ions, which exhibited statistically significant differences in peak intensity between average environmental and clinical mass spectra using ClinProTools software. Six out of ten mass ions were assigned as environmental-identifying biomarker ions (EIBIs), including, m/z 4,056, 4,214, 5,814, 7,545, 7,895, and 8,112, whereas the remaining four mass ions were defined as clinical-identifying biomarker ions (CIBIs) consisting of m/z 3,658, 6,322, 7,035, and 7,984. Hence, our findings represented, for the first time, the source-specific biomarkers of environmental and clinical B. pseudomallei. © 2014 Niyompanich et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902590136&doi=10.1371%2fjournal.pone.0099160&partnerID=40&md5=c6a3397c7511fb7cc77abcc0a0f8a8c5
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Source-identifying biomarker ions between environmental and clinical Burkholderia pseudomallei using whole-cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
Niyompanich S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia specie Utilization of whole-cell MALDI-TOF mass spectrometry to differentiate Burkholderia pseudomallei wild-type and constructed mutants การพัฒนาวิธีเตรียมตัวอย่างเลือดเพื่อจำแนกเชื้อแบคทีเรียโดยตรงด้วย MALDI-TOF MS Development of blood sample preparation method for direct bacterial identification using MALDI-TOF MS ผลของสภาวะการเพาะเลี้ยงต่อการจำแนกเชื้อบาซิลลัสแอนทราซิสด้วยเทคนิค MALDI-TOF MS Effects of culture conditions for Bacillus anthracis identification based on MALDI-TOF MS An economic evaluation of neonatal screening for inborn errors of metabolism using tandem mass spectrometry in Thailand การวิเคราะห์สารอัลคาลอยด์มัสคาลีนในเห็ดพิษด้วยวิธี Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) An Analysis of alkaloid muscarine-containing mushrooms using liquid chromatography-tandem m Soil nutrient depletion is associated with the presence of Burkholderia pseudomallei Immune response to recombinant Burkholderia pseudomallei FliC Hevea B Serum Proteome Profiling Using Liquid Chromatography-Mass Spectrometry Elemental imaging and classifying rice grains by using laser ablation inductively coupled plasma mass spectrometry and linear discriminant analysis
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