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Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus
Jariyapong P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus
ผู้แต่ง / หัวหน้าโครงการ (EN): Jariyapong P.
บทคัดย่อ (EN): We tested the use of Macrobrachium rosenbergii nodavirus-like particles (MrNv-VLPs) as a delivery mechanism to carry therapeutic agents against white spot syndrome in shrimp. We used constructed double-stranded RNA called VP28 (VP28 dsRNA) against WSSV envelope genes to confer protection against the pathogen. Results showed that MrNv-VLP was able to encapsulate VP28 dsRNA. Using enhanced green fluorescent protein (EGFP) as a reporter, we found that VLP penetrated various shrimp tissues including the muscle, hepatopancreas, and gill. A statistically significant relative survival rate of 44.5% was obtained in the group of shrimp receiving encapsulated VP28 dsRNA-VLP after WSSV challenge as compared to 100% mortality in the control shrimp at 7. days post-infection. Shrimp treated with EGFP dsRNA loaded into MrNv-VLPs showed relatively similar motility rates as those of controls. Moreover, MrNv-VLP encapsulation improved VP28 dsRNA efficiency against WSSV. A higher survival rate of 16.7% was observed in the group of shrimp receiving encapsulated VP28 dsRNA-VLP when compared to those receiving naked VP28 dsRNA. These results indicate that MrNv-VLP is a good candidate for use as a therapeutic delivery system against shrimp diseases due to its self-reassembly property, broad target of various shrimp tissues and immune enhancement. © 2014 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908431508&doi=10.1016%2fj.aquaculture.2014.09.034&partnerID=40&md5=0c17f02ca9c13c554cb62c052b6b585d
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Shrimp white spot syndrome virus
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus
Jariyapong P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Use of microalgae Chlamydomonas reinhardtii for production of double-stranded RNA against shrimp virus ICP35 is a TREX-like protein identified in white spot syndrome virus Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment Shrimp hemocyte homeostasis-associated protein (PmHHAP) interacts with WSSV134 to control apoptosis in white spot syndrome virus infection Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp
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