สืบค้นงานวิจัย
Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa
Buranaamnuay K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa
ผู้แต่ง / หัวหน้าโครงการ (EN): Buranaamnuay K.
บทคัดย่อ (EN): Frozen semen of eight bulls was used to assess effects of storage temperature and length of storage time on frozen-thawed bovine sperm quality. In experiment 1, 25 straws of frozen semen of each bull were allocated to 3 groups. The control was still maintained in liquid nitrogen (LN2). The rest were abruptly moved from LN2 to −80 °C and −30 °C mechanical freezers, respectively. After thawing, it was found that the sperm motility, vitality and membrane integrity were comparable (P > 0.05) between the control and the −80 °C samples and were significantly inferior (P < 0.001) in the −30 °C samples, irrespective of storage time (1-day, 1-week and 1-month). In experiment 2, two out of the three parts (16–18 straws) of frozen semen of each bull were rapidly removed from LN2 and further kept in the freezer (−80 °C). One day before being thawed, half of the samples in the freezer were promptly put back to LN2. The results showed that the frozen-thawed sperm quality was not significantly affected (P > 0.05) both by storage temperature (−196 °C, −80 °C and −80 & −196 °C) and storage time [day-2, day-8 (1-week) and day-31 (1-month)]. At the same storage times, the quality measures at different temperatures were not significantly different from one another (P > 0.05). In conclusion, a −80 °C mechanical freezer was as effective as LN2 in preserving in vitro quality of frozen-thawed bovine spermatozoa throughout 1-month of storage. When required for use, frozen semen stored in the freezer could be thawed immediately or transferred to the LN2 tank for thawing elsewhere. © 2016 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990061224&doi=10.1016%2fj.anireprosci.2016.06.011&partnerID=40&md5=933cce492f8cb95e5f0cf29f1512b05f
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Time Factors
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Buranaamnuay K.. "Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa". ไม่ระบุหน่วยงาน. ม.ป.ป.

Buranaamnuay K.. "Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa
Buranaamnuay K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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