คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Uranan Tumkosit

เผยแพร่โดย

1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 3 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 5 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan. Electronic address: washi69@biken.osaka-u.ac.jp.

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งานวิจัยที่แนะนำ Characterization of chikungunya virus-like particles Characterization of Chikungunya Virus-Like Particles Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus Polyacrylamide hydrogel encapsulated E. coli expressing metal-sensing green fluorescent protein as a potential tool for copper ion determination Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection In vitro neutralization of yellow head virus infection in shrimp using recombinant PmYRP65 protein Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus ICP35 is a TREX-like protein identified in white spot syndrome virus Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1

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ชื่อเรื่อง (EN):	The use of green fluorescent protein-tagged virus-like particles as a tracer in the early phase of chikungunya infection
ผู้แต่ง / หัวหน้าโครงการ (EN):	Uranan Tumkosit
บทคัดย่อ (EN):	To visually examine the early phase of chikungunya virus (CHIKV) infection in target cells, we constructed a virus-like particle (VLP) in which the envelope protein E1 is fused with green fluorescent protein (GFP). This chikungunya VLP-GFP (CHIK-VLP-EGFP), purified by density gradient fractionation, was observed as 60-70 nm-dia. particles and was detected as tiny puncta of fluorescence in the cells. CHIK-VLP-EGFP showed binding properties similar to those of the wild-type viruses. Most of the fluorescence signals that had bound on Vero cells disappeared within 30 min at 37°C, but not in the presence of anti-CHIKV neutralizing serum or an endosomal acidification inhibitor (bafilomycin A1), suggesting that the loss of fluorescence signals is due to the disassembly of the viral envelope following the internalization of CHIK-VLP-EGFP. In addition to these results, the fluorescence signals disappeared in highly susceptible Vero and U251MG cells but not in poorly susceptible A549 cells. Thus, CHIK-VLP-EGFP is a useful tool to examine the effects of the CHIKV neutralizing antibodies and antiviral compounds that are effective in the entry phase of CHIKV.
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ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=565
เผยแพร่โดย (EN):	1 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 3 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 5 Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan. Electronic address: washi69@biken.osaka-u.ac.jp.
คำสำคัญ (EN):	Virus-like particle
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science