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Multilocus sequence typing of P. multocida isolates of buffalo origin from Gujarat state of India (Buffalo Bulletin (Thailand),36(2))
Ashish Roy - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Multilocus sequence typing of P. multocida isolates of buffalo origin from Gujarat state of India (Buffalo Bulletin (Thailand),36(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Ashish Roy
หน่วยงานสังกัดผู้แต่ง (EN): Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Microbiology
บทคัดย่อ (EN): Haemorrhagic septicaemia in Indian buffaloes is mainly caused by Pasteurella multocida serotype B: 2. The present study was conducted to characterize the P. multocida serotype B: 2 isolates of buffalo origin by multilocus sequence typing (MLST) to identify the sequence types prevailing around Gujarat state in India. A total of seven field isolates collected from different regions across the state and a reference strain (P52) were used for this study. Seven housekeeping genes were specifically amplified by PCR; the gene sequences obtained were trimmed to specific length and allelic profiles were assigned to these seven loci. The assigned allelic profiles were 23, 37, 21, 17, 4, 2 and 17 for adk, est, pmi, zwf, mdh, gdh and pgi genes, respectively and all the isolates were grouped in one sequence type, sequence type 122 (ST122). eBURST analysis classified ST122 into group number 23 when group definition was 5 or more matches. But when group definition was 6 or more matches it was classified into group number 22 along with ST63. Phylogenetic and Splits Tree analysis showed that ST122 is closely related to ST63, ST127, ST162 and ST147 and all five of them were grouped in a cluster. From the study it was concluded that ST122 may be the predominant sequence type in buffaloes of Gujarat state.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/368845
คำสำคัญ (EN): Allelic profile
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Multilocus sequence typing of P. multocida isolates of buffalo origin from Gujarat state of India (Buffalo Bulletin (Thailand),36(2))
Ashish Roy
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Characterization of Pasteurella multocida isolates of buffalo origin from Gujarat state of India by outer membrane protein profile analysis (Buffalo Bulletin (Thailand),36(2)) Study of buffalo husbandry practices in rural area of central Gujarat in India (Buffalo Bulletin (Thailand),36(1)) Oesophageal obstruction in a pregnant buffalo (Buffalo Bulletin (Thailand),36(4)) Ethno-veterinary treatment of buffalo in Arid region of Rajasthan, India (Buffalo Bulletin (Thailand),36(2)) Seasonal variation in the characteristics of the swamp buffalo semen of northeast India (Buffalo Bulletin (Thailand),36(1)) Karyotype of Mazani water buffalo from Iran (Buffalo Bulletin (Thailand),36(1)) Prevalence of cryptosporidiosis in buffalo calves of Jabalpur, India (Buffalo Bulletin (Thailand),37(1)) Dystocia due to foetal ascites in a Murrah buffalo (Buffalo Bulletin (Thailand),36(3)) Phenotypic characteristics of Sambalpuri buffaloes of India (Buffalo Bulletin (Thailand),36(4)) Isolation of hippuric acid from buffalo urine and its antioxidant activity (Buffalo Bulletin (Thailand),36(3))
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