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Proteomic analysis and white spot syndrome virus interaction of mud crab (Scylla olivacea) revealed responsive roles of the hemocytes
Havanapan P.-O. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Proteomic analysis and white spot syndrome virus interaction of mud crab (Scylla olivacea) revealed responsive roles of the hemocytes
ผู้แต่ง / หัวหน้าโครงการ (EN): Havanapan P.-O.
บทคัดย่อ (EN): White spot disease (WSD) is a highly virulent viral disease in shrimps. Clinical signs and high mortality of WSD is generally observed after a few days of infection by White Spot Syndrome virus (WSSV). Mud crabs are the major carrier and persistent host for the WSSV. However, an elucidation of viral interaction and persistent mode of WSSV infection in mud crab is still limited. We investigated the defensive role of mud crab (Scylla olivacea) hemocytes against WSSV infection by using comparative proteomic analysis coupled with electrospray ionization liquid chromatography tandem mass spectrometry (ESI-LC/MS/MS). The proteomic maps of expressed proteins obtained from WSSV infected hemocytes revealed differential proteins related to various biological functions, including immune response, anti-apoptosis, endocytosis, phosphorylation signaling, stress response, oxygen transport, molting, metabolism, and biosynthesis. Four distinctive cell types of crab hemocytes: hyaline cells (HC), small granular cells (SGC), large granular cells (LGC) and mixed granular cells (MGC) were found susceptible to WSSV. However, immunohistochemistry analysis demonstrated a complete replication of WSSV only in SGC and LGC. WSSV induced apoptosis was also observed in HC, SGC and MGC except for LGC. These results suggested that HC and MGC may undergo apoptosis prior to a complete assembly of virion, while SGC is more susceptible showing higher amplification and releasing of virion. In contrast, WSSV may inhibit apoptosis in infected LGC to stay in latency. This present finding provides an insight for the responsive roles of crustacean hemocyte cells involved in molecular interaction and defense mechanism against WSSV. © 2019 Elsevier Ltd
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064176041&doi=10.1016%2fj.fsi.2019.03.070&partnerID=40&md5=2fff38e833f8367e95494dc48ff89821
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): White spot syndrome virus
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Proteomic analysis and white spot syndrome virus interaction of mud crab (Scylla olivacea) revealed responsive roles of the hemocytes
Havanapan P.-O.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture ICP35 is a TREX-like protein identified in white spot syndrome virus Identification of genes associated with reproduction in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation Identification and expression of prostaglandin E synthase (PGES) gene in the central nervous system and ovary during ovarian maturation of the female mud crab, Scylla olivacea Distribution of crustacean hyperglycemic hormones (CHH) in the mud crab (Scylla olivacea) and their differential expression following serotonin stimulation Shrimp hemocyte homeostasis-associated protein (PmHHAP) interacts with WSSV134 to control apoptosis in white spot syndrome virus infection Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon ความสัมพันธ์ระหว่างสิ่งแวดล้อมและการเกิดโรคไวรัสตัวแดงดวงขาว (White spot syndrome Virus, WSSV) ในกุ้งกุลาดำ Yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (WSSV) in shrimp ความสัมพันธ์ระหว่างปริมาณเชื้อไวรัสตัวแดงดวงขาว (White spot syndrome virus, WSSV) กับการถ่ายทอดโรคในกุ้งกุลาดำโดยใช้เทคนิค real time PCR
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