สืบค้นงานวิจัย
The hematopoietic organ of Macrobrachium rosenbergii: Structure, organization and immune status
Pudgerd A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): The hematopoietic organ of Macrobrachium rosenbergii: Structure, organization and immune status
ผู้แต่ง / หัวหน้าโครงการ (EN): Pudgerd A.
บทคัดย่อ (EN): The hematopoietic organ (HO) of the giant freshwater prawn Macrobrachium rosenbergii is a discrete, whitish mass located in the epigastric region of the cephalothorax, posterior to the brain. It is composed of hematopoietic cells arranged in a thick layer of numerous lobules that surround a central hemal sinus from which they are separated by a thin sheath. At the center of the sinus is the muscular cor frontale. The lobules extend radially outward from the sinus in three developmental zones. Basal Zone 1 nearest the sinus contains large hematopoietic stem cells with euchromatic nuclei that stain positive for proliferation cell nuclear antigen (PCNA). Zone 2 contains smaller, actively dividing cells as indicated by positive 5-bromo-20-deoxyuridine (BrdU) staining. Distal Zone 3 contains small, loosely packed cells with heterochromatic nuclei, many cytoplasmic granules and vesicles indicating that they will eventually differentiate into hemocytes and enter circulation. Three main arteries, namely the ophthalmic and the 2 branches of the antennary, connect the heart to the HO. Use of India ink and 0.1 μm fluorescent micro-beads injected into the heart revealed that the cor frontale could immediately remove foreign particles from hemolymph by filtration. Fluorescent beads were also detected in the hematopoietic tissue at 30 min after injection, indicating that it could be penetrated by foreign particles. However, the fluorescent signal completely disappeared from the whole HO after 4 h, indicating its role in removal of foreign particles. In conclusion, the present study demonstrated for the first time the detailed histological structures of the HO of M. rosenbergii and its relationship to hematopoiesis and removal of foreign particles from hemolymph. © 2019 Elsevier Ltd
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062809336&doi=10.1016%2fj.fsi.2019.03.011&partnerID=40&md5=cc52f811937f9ae2e837c775524cef90
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): tissue structure
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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The hematopoietic organ of Macrobrachium rosenbergii: Structure, organization and immune status
Pudgerd A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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