สืบค้นงานวิจัย
Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis
Yamkamon V. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis
ผู้แต่ง / หัวหน้าโครงการ (EN): Yamkamon V.
บทคัดย่อ (EN): An enzyme-coupled colorimetric assay for quantification of urinary sarcosine was developed. The proposed method is a specific reaction based on hydrogen peroxide (H2O2) formation via sarcosine oxidase (SOX). The liberated H2O2 reacts with Amplex Red in the presence of horseradish peroxidase (HRP) to produce the redfluorescent oxidation product, resorufin, which can be measured spectrophotometrically (OD570). The method was performed in the 96-well microtiter plate. Reaction conditions, such as pH and reaction time were optimized. At the optimum conditions, the limit of detection (LOD) and quantification (LOQ) were found to be 0.7 and 1 μM, respectively. A good linearity was revealed with a coefficient of 0.990. The assay showed no significant interference from ascorbic acid, glucose and bilirubin. In addition, it is extremely specific for sarcosine rather than other amino acids. The determination of sarcosine in human urine displayed high accuracy and good reproducibility. This method is promising to differentiate prostate cancer patients from healthy subjects according to urinary sarcosine level. Altogether, this study provides a rapid, simple and specific tool to determine urinary sarcosine which could be useful for prostate cancer diagnosis. © 2018, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051559140&doi=10.17179%2fexcli2018-1245&partnerID=40&md5=0b735f0d814e99ab3b0a1096d7cfba82
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): urine level
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Yamkamon V.. "Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Yamkamon V.. "Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis
Yamkamon V.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Simultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosis Novel method for the determination of tetracycline antibiotics in bovine milk based on digital-image-based colorimetry Molecular diagnosis of α-thalassemias by the colorimetric nanogold Development and validate method for zoonoses test kits Development and Validation of an Enzymatic Method to Determine Stevioside Content from Stevia rebaudiana Rubber-Based Farming System: in a Challenging Industry Development and Expansion in Southern Philippines Development of molecular approach based on PCR assay for detection of histamine producing bacteria The development of the cloaca in the human embryo Re-evaluation of generic characters for Blinkworthia (Convolvulaceae) based on morphology and reproductive organ development The model for Thais physician competency development based on Focus Discussion Group (FDG): A review
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair