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Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials
Nittaya Methawanitpong - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials
ผู้แต่ง / หัวหน้าโครงการ (EN): Nittaya Methawanitpong
บทคัดย่อ (EN): Introduction: Culex mosquitoes are commonly found in Thailand. These mosquitoes are not only irritate people and animals at night, but they also spread mosquito-borne diseases through the bites of infected mosquitoes. Culex quinquefasciatus and Culex tritaeniorhynchus are considered as the principal vectors of Lymphatic filariasis (LF) and Japanese encephalitis (JE), respectively. Currently finding many migrants from endemic neighboring countries, especially Mynmar carrying mosquito-borne diseases include LF and JE, thus there is a high possibility that those diseases could be spread to Thai people. The use of temephos (chemical larvicide) is one of the main strategies to control Culex larvae along with other mosquito control methods. However, using alternatives to chemical insecticides such as Bacillus thuringiensis subsp. israelensis (Bti) as a biological control agent, it is safe to humans and environment. Further, Bti plays an important role in temephos resistance in mosquito larvae. This microbial agent produces a protein crystal that is specific toxic to larvae of Culex, Aedes and Anopheles spp. Unfortunately, Bti agent is difficult to order from domestic suppliers, it need to be ordered from oversea companies with the high prices. Therefore, it is best that to find appropriate media with a low cost for culture of Bti by using cheap and available waste materials. Objectives: 1. To evaluate three different culture media (soy pulp, mature coconut water and rice rinse water) for production of Bti 2. To evaluate larvicidal efficacy of the Bti obtained from the best culture medium against Culex spp. larvae under field conditions. Methodology: Bti strain PR2502 was isolated from soil samples collected from Prae province. Bti strain was maintained on nutrient agar plates at 4°C prior to the tests. Bti was grown in each of three studied culture (soy pulp, mature coconut water and rice rinse water) with shaking 200 rpm for 48 hours at room temperature. The most cost-effective culture medium with the lowest LC50 value (the lethal concentration 50: concentration required to kill 50% of the test mosquitoes) was selected. Biomass production of the Bti was conducted in a 5-litre plastic container with lid containing 10 g/l selected medium and 0.2 g/l Bti powder with thoroughly stirred. The incubation period was 48 hours at room temperature with aeration provided by two set of air pump and air-stone to ensure bacterial growth. The Bti was tested for its efficacy in the laboratory against Cx. quinquefasciatus and also conducted against natural larvae of Culex spp. in Nonthaburi, Nakhonpathom and Samutsakhon provinces. For a comparison of the cost-effectiveness among the three medium cultures for Bti productions was done by using Duncan's multiple range test (P≤0.05). Results: LC50 values of Bti cultured from soy pulp, mature coconut water and rice rinse water against laboratory strain of Cx. quinquefasciatus larvae were 3.94, 18.21 and 23.65 ppm, respectively. The soy pulp was found to be the best culture medium for Bti with the lowest LC50 value. For field tests, efficacy of the Bti from soy pulp could help reduce dramatically of Culex spp. larvae (~90%) in natural breeding sites in Nonthaburi, Nakhonpathom and Samutsakhon provinces using Bti 0.5, 0.7 and 1 litre/m2, respectively with a minimum persistence of two weeks. Conclusion: The soy pulp was considered the most cost-effective culture medium for the production of Bti toxin compared with mature coconut water and rice rinse water. Thus, the soy pulp can be used as alternative to cheap culture medium for growth and spore formation of Bti. Moreover, this knowledge can be transferred to local communities toencourage self-reliance and participation for mosquito control activities.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=628
เผยแพร่โดย (EN): National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
คำสำคัญ (EN): Biological Contro
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Nittaya Methawanitpong. "Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials". ไม่ระบุหน่วยงาน. ม.ป.ป.

Nittaya Methawanitpong. "Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials
Nittaya Methawanitpong
National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Thailand
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การประเมินผล Bacillus thuringiensis subsp. Israelensis ที่เพาะเลี้ยงด้วยวัสดุเหลือทิ้งในการกำจัดลูกน้ำยุงรำคาญ Evaluation of Bacillus thuringiensis subsp. Israelensis produced from waste product for c Behavioral Action of Deltamethrin and Cypermethrin in Pyrethroid-Resistant Aedes aegypti (Diptera: Culicidae): Implications for Control Strategies in Thailand Efficiency, Toxicity and Persistence of Temephos (Sand Granule), Bacillus thuringiensis var. israelensis and Novaluron on Aedes aegypti L. Larvae in Sawaeng Ha district, Ang Thong province การทดสอบผลผลิตและส่วนประกอบทางเคมีของหญ้าสกุล Brachiaria spp.ในพื้นที่จังหวัดมุกดาหาร Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefascia Antifungal metabolites of Aspergillus spp. and Trichoderma spp. as a biological control measures against Rigidoporus microporus Monitoring of resistance in laboratory susceptible strain and field population strains of Culex quinquefasciatus to Bacillus thuringiensis var. israelensis (Bti), Bacillus sphaericus (Bsph) and temeph ผลของระยะเวลาตัดที่มีต่อผลผลิตถั่ว Aeschynomene spp. ในพื้นที่ลุ่ม การตรวจจำแนกเชื้อ Bacillus cereus และ Bacillus thuringiensis ด้วยเทคนิค multiplex PCR Differentiation of Bacillus cereus and Bacillus thuringiensis by multiplex PCR assay Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae
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