สืบค้นงานวิจัย
Generation of porcine induced-pluripotent stem cells from Sertoli cells
Setthawong P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Generation of porcine induced-pluripotent stem cells from Sertoli cells
ผู้แต่ง / หัวหน้าโครงการ (EN): Setthawong P.
บทคัดย่อ (EN): Induced pluripotent stem cells (iPSCs) are generated by reprogramming of somatic cells using four transcription factors: OCT4, SOX2, KLF-4, and c-MYC (OSKM). However, reprogramming efficiency of iPSCs is currently poor. In this study, we used the Sertoli line as a novel cell source for somatic cell reprogramming. Neonatal testes were collected from 1-week-old piglets. The testes were digested by a two-step enzymatic method to isolate Sertoli cells. The latter were transfected with retroviral vectors expressing OSKM. The Sertoli iPSC-like colonies were subjected to morphological analysis, alkaline phosphatase staining, RT-PCR, G-banding karyotyping, in vitro differentiation, and in vivo differentiation. Primary Sertoli cells had polygon-shaped morphology and manifested phagocytic activity as determined by a fluorescent bead assay. Sertoli cells also expressed the anti-Müllerian hormone protein in the cytoplasm. According to RT-PCR results, these cells expressed Sertoli cell markers (FSHR, KRT18, and GATA6) and endogenous transcription factors genes (KLF4 and c-MYC). A total of 240 colonies (0.3% efficiency) were detected by day 7 after viral transduction of 72500 cells. The Sertoli iPSC-like colonies contained small cells with a high nucleus-to-cytoplasm ratio. These colonies tested positive for alkaline phosphatase staining, expressed endogenous pluripotency genes, and had a normal karyotype. All these cell lines could form in vitro three-dimensional aggregates that represented three germ layers of embryonic-like cells. A total of two cell lines used for in vivo differentiation produced high-efficiency teratoma. In conclusion, Sertoli cells can efficiently serve as a novel cell source for iPSC reprogramming. © 2018
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060328060&doi=10.1016%2fj.theriogenology.2018.12.033&partnerID=40&md5=d0dfded89f3029a205369d1a6b579717
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Transfection
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Setthawong P.. "Generation of porcine induced-pluripotent stem cells from Sertoli cells". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Generation of porcine induced-pluripotent stem cells from Sertoli cells
Setthawong P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation In vitro production of functional immune cells derived from human hematopoietic stem cells Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs) Computational identification of miRNAs that modulate the differentiation of mesenchymal stem cells to osteoblasts Therapeutic benefit of intra-articular administration of deciduous teeth stem cells in rabbit model of osteoarthritis Enhancement of TNF-α induced antiproliferation in human cervical cancer cells by extracts from Thai medicinal plants Selective Estrogen Receptor Modulator (SERM)-like Activities of Diarylheptanoid, a Phytoestrogen from Curcuma comosa, in Breast Cancer Cells, Pre-osteoblast Cells, and Rat Uterine Tissues Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Histone modification levels in β-thalassemia/hemoglobin E erythroid cells
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