สืบค้นงานวิจัย
Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization
Lekakarn H. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization
ผู้แต่ง / หัวหน้าโครงการ (EN): Lekakarn H.
บทคัดย่อ (EN): The binary toxin produced by Lysinibacillus sphaericus is composed of BinA and BinB subunits. Together, but not separately, the two subunits are highly toxic to Culex quinquefasciatus larvae, but show no toxicity to Aedes aegypti. The molecular mechanism underlying intoxication has not been clearly elucidated. The present study compares the binding and the internalization of binary toxin into the midgut epithelial cells of susceptible C. quinquefasciatus mosquito larvae with those of Bin-refractory A. aegypti. The guts from larvae fed with fluorescently labeled toxin were dissected and analyzed using a confocal laser scanning microscope. When fed with a mixture of both components, co-localization of BinA and BinB was detected both on the cell surface and in the cytoplasm of Culex larval gut cells. However, administration of BinA alone resulted in localization only on the cell membrane, whereas BinB alone was detected both on the cell membrane and inside the cytoplasm. In contrast, when a mixture of both components, or each individual component, was fed to Aedes larvae, BinA and BinB were unable to reach the cytoplasm and were localized only on the cell membrane. These results are consistent with the suggestion that the internalization of BinA is essential for toxicity, and that BinB is required for this internalization into susceptible larval gut cells. © 2015 Elsevier Inc.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943388478&doi=10.1016%2fj.jip.2015.09.010&partnerID=40&md5=225e26078a4169c810b9ae7823990d51
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Larva
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Lekakarn H.. "Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization". ไม่ระบุหน่วยงาน. ม.ป.ป.

Lekakarn H.. "Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization
Lekakarn H.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Binary toxin subunits of lysinibacillus sphaericus are monomeric and form heterodimers after in vitro activation Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes Development of B-Soy starter for biolarvicide production for control of Culex quinquefasciatus mosquito larvae Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefascia Changes in lipid metabolism convey acid tolerance in Saccharomyces cerevisiae 06 Biological Sciences 0601 Biochemistry and Cell Biology In vitro generation of dendritic cell - breast cancer cell hybrids for induction of cellular immunity The C-terminal domain of the Bacillus thuringiensis Cry4Ba mosquito-specific toxin serves as a potential membrane anchor
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