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Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes
Havanapan P.-O. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes
ผู้แต่ง / หัวหน้าโครงการ (EN): Havanapan P.-O.
บทคัดย่อ (EN): Our previous data revealed that viral particles of yellow head virus (YHV) specifically interacted with granule-containing hemocytes. After isolation of targeted hemocytes, biotinylation was performed using Biotin-NSH-LC. Biotinylated protein was extracted and separated by 2-D PAGE. Electro-transferred proteins on a nitrocellulose membrane were probed with streptavidin-HRP complex to detect biotinylated proteins. The data from 2-D PAGE combined with affinity pull down purification revealed 8 and 6 biotinylated proteins specific to hyaline and granule containing hemocytes, respectively. Four proteins were found in common for both two hemocytes. The majority of proteins detected in granular hemocytes are membrane-associated proteins and immune-related proteins such as alpha-2-macroglobulin (A2M), kazal-type serine protease inhibitor (SPI) and crustin. Crustin. Pm1 was found to bind to YHV as shown with biotinylation pull-down assay and confirmed with two-dimensional virus overlay protein binding assay (2-D VOPBA). The expression of crustin. Pm1 was observed in semigranular and granular hemocytes whereas very low or no expression occurred in hyaline hemocytes. Crustin. Pm1 appears to either be directly involved in cellular binding or mediating virus internalization into permissive hemocytes. © 2014 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907689141&doi=10.1016%2fj.fsi.2014.08.014&partnerID=40&md5=a54a8d48c8ddb906dd0f834ab8c2557a
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Virus Attachment
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes
Havanapan P.-O.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon) Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon Correlation between gonad-inhibiting hormone and vitellogenin during ovarian maturation in the domesticated Penaeus monodon In vitro neutralization of yellow head virus infection in shrimp using recombinant PmYRP65 protein Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon Cell surface transglutaminase required for nodavirus entry into freshwater prawn hemocytes
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