คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Kritchai Poonchareon

เผยแพร่โดย

1 Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao Province, Thailand. 2 Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Nonthaburi Province, Thailand. 3 Phayao Ram Hospital, Phayao Province, Thailand 4 Integrative Research Center for Veterinary Preventive Medicine, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai Province, Thailand.

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งานวิจัยที่แนะนำ Rapid detection of tuberculosis resistant to isoniazid and rifampicin by real-time PCR and melting curve analysis การตรวจชนิดของเชื้อพยาธิที่มีเห็บเป็นพาหะในเลือดสุนัขด้วยเทคนิค high resolution melting analysis การพัฒนาชุดไพรเมอร์เพื่อการตรวจคัดแยกการติดเชื้อวัณโรคในเป็ดด้วยเทคนิค Multiplex real-time PCR ควบคู่กับ High-resolution melting analysis High-Resolution Single-Cell Sequencing of Malaria Parasites Phylogenetic analysis reveals two morphologically unique new species in the genera Astrochapsa and Nitidochapsa (lichenized Ascomycota : Graphidaceae) การพัฒนาเทคนิค High resolution melting real-time PCR เพื่อตรวจหารูปแบบยีน 24BP-PRL ในไก่พื้นเมืองไทยพันธุ์ประดู่หางดำ Rapid clinical assessment to facilitate the triage of adults with falciparum malaria, a retrospective analysis Relationship between PM10 and PM2.5 levels in high-traffic area determined using path analysis and linear regression Salmonella enterica multilocus sequence typing and its correlation with serotypes Analysis of Novel Mutations and Methylmalonyl-CoA Mutase Levels in Thai Patients with Isolated Methylmalonic Acidemia

คัดลอก URL

ชื่อเรื่อง (EN):	Feasibility of high resolution melting curve analysis for rapid serotyping of Salmonella from hospitalised patients
ผู้แต่ง / หัวหน้าโครงการ (EN):	Kritchai Poonchareon
บทคัดย่อ (EN):	Backgrounds: Serum agglutination test is the gold standard phenotyping method widely used for Salmonella enterica characterisation.This conventional method is limited by its complicated and time-consuming procedures. High resolution melting curve (HRM) analysis is introduced as a rapid and labour-saving method. Objectives: To compare the results of conventional serum agglutination and quantitative PCR-HRM analysis to assess the feasibility of this alternative approach for Salmonella serotyping. Materials and methods: Salmonella strains from 38 human-originating samples were serotyped using the conventional serum agglutination method and HRM analysis. Results: The conventional serum agglutination assay detected 14 serotypes, while the HRM analysis identified 10 HRM profiles. There was a correlation between most of the serotyping results obtained by the two methods. Nine of the HRM profiles were unique to a single serotype, of each. One exception was HRM_3. Many of the indistinct curves that were grouped in this HRM pattern belonged to five Salmonella serotypes, including Weltevreden, Corvallis, Derby, Kedougou and Kentucky. Conclusion: It is difficult to determine all Salmonella serotypes by HRM analysis. However, this method can be used as an alternative to the conventional serum agglutination assay for rapid and labour-saving serotyping.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=539
เผยแพร่โดย (EN):	1 Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao Province, Thailand. 2 Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Nonthaburi Province, Thailand. 3 Phayao Ram Hospital, Phayao Province, Thailand 4 Integrative Research Center for Veterinary Preventive Medicine, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai Province, Thailand.
คำสำคัญ (EN):	Salmonella; Serotyping; HRM analysis
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science