สืบค้นงานวิจัย
THE EFFECTOR OF CORYNESPORA CASSIICOLA VIRULENCE IN RUBBER TREE
ValériePujade–Renaud - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): THE EFFECTOR OF CORYNESPORA CASSIICOLA VIRULENCE IN RUBBER TREE
ผู้แต่ง / หัวหน้าโครงการ (EN): ValériePujade–Renaud
บทคัดย่อ (EN): Corynespora cassiicola, causing agent of the Corynespora Leaf Fall (CLF) of rubber tree, is an Ascomycete Fungusable to infect a large of range of plants, mostly in tropical areas or green houses. Over the Past 20 years, the CLF disease has gained increasing importance in all Asian and African rubber producing countries but has not been declared so far in South America. It causes massive sporadic leaf falls, thus impacting the yield of rubber production. Susceptibility of the various rubber clones to the disease varies depending on the geographical location, suggesting an important diversity of the natural inoculum. The biology of the fungus is complex since it has been described as a necrotrophic pathogen as well as an endophyte or a saphophyte. In this presentation, I will give a broad description of the research program that we are developing for the characterization of fungal effectors involved in the H. brasiliensis/C. cassiicola interaction. Our objective is to describe the diversity of the C. cassiicola species, trying to connect the genetic races with specific effectors potentially involved in virulence or host specialization of the isolates. In this program, we have first used biochemical and molecular approaches, which lead to the characterization of the cassiicolin toxin, a small secreted protein (SSP) putatively involved in the pathogenicity of C. cassiicola (isolate CCP) (Bartheet al., 2007; Breton, Sanier, & d’ Auzac, 2000; de Lamotte et al., 2007; Déonet al. 2012a) A phylogenetic analysis based on C. cassiicola isolates of various geographical origins and host plants identified at least 8 phylogenetic clades (Déonet al. 2014). In addition, a classification based on the cassiicolin gene diversity was proposed (toxin classes). We found a rather good coherence between the toxin classes and phylogenetic clades. Howerver, 53% of the isolates did not carry any cassicolin gene, although some of them are virulent, suggesting the existence of effectors other than cassiicolin. This analysis is currently being extended to new isolates recently collected in West Africa (preliminary results will be presented). We are also conducting a genomic/transcriptomic approach with the objective to identify all putative effectors involved in the interaction with rubber tree. We have obtained the first reference sequence of the C. cassiicola genome, in frame of the DOE – JGI “1000 Fungal Genome” program. The assembled genome is around 45 Mb and contains about 17000 genes. We are now conducting comparative genomic analysis. The analysis pipeline is presented below. Interspecific comparative genomics with 45 fungal genome, mostly pathogensfrom the Dothideomycetes (Schoch et al., 2009), aims to describe the potential pathogenesis effectors arsenal, incouding the secondary metabolites, SSPs, lipases, proteases and CAZymes. Intraspecific comparative genomics among 37 C. cassiicola isolates of our collection, covering the known diversity, will help defining new and complete effector patterns that will be analyzed comparatively togenome – wide phylogenetic profiles. Finally, a transcriptomic analysis (RNAseq) of fungal genes differentially expressed after inoculation of detached rubber tree leaves (sensitive clone PB 260) with a spore suspension (isolate CCP) is being conducted, in order to identify functional and pertinent effector candidatesm. The best candidates will be validated by functional genetics (through the knock – down of effector encoding genes). This study will provide knowledge and molecular tools for the characterization of C. cassiicola populations in epidemiological studies. This knowledge will help adapting the breeding strategies, taking into account the diversity of the pathogen, for the selection of more tolerant rubber tree clones.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://agritrop.cirad.fr/579208/
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): trancriptomics.
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

ValériePujade–Renaud. "THE EFFECTOR OF CORYNESPORA CASSIICOLA VIRULENCE IN RUBBER TREE". ไม่ระบุหน่วยงาน. ม.ป.ป.

ValériePujade–Renaud. "THE EFFECTOR OF CORYNESPORA CASSIICOLA VIRULENCE IN RUBBER TREE". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
THE EFFECTOR OF CORYNESPORA CASSIICOLA VIRULENCE IN RUBBER TREE
ValériePujade–Renaud
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
DIVERSITY OF THE CASSIICOLIN GENE IN CORYNESPORA CASSIICOLA OF RUBBER TREE IN CHINA PATHOGENICITY ASSAY OF CORYNESPORA CASSIICOLA ISOLATES FROM RUBBER TREE AND OTHER HOSTS IN VIETNAM Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf GENETIC MAPPING AND IDENTIFICATION OF QTLS INVOLVED IN THE TOLERANCE OF RUBBER TREE (HEVEA BRASILIENSIS) TO CORYNESPORA CASSIICOLA SECRETED EFFECTRORS Diversity Analysis of Corynespora cassiicola Isolated from Indonesian Rubber Plantations Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN ANALYSIS OF PRETREATMENT METHODS OF WASTE RUBBER TREE FOR EFFECTIVE CELLULOSE DEGRADATION Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis) Chloroplast DNA Microsatellite Differentiation in Cultivated and Wild Rubber Tree (Hevea brasiliensis)
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair