สืบค้นงานวิจัย
A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon
Leebonoi W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon
ผู้แต่ง / หัวหน้าโครงการ (EN): Leebonoi W.
บทคัดย่อ (EN): Argonaute is a key protein of the small-RNA guided gene regulation process. The Argonaute family is generally divided into two subfamilies; AGO and PIWI. In this study, a cDNA encoding a novel type of Argonaute (PmAgo4) in the black tiger shrimp Penaeus monodon was identified and characterized. PmAgo4 cDNA contained an open reading frame of 2433 nucleotides that can be translated into a deduced amino acid with the conserved PAZ and PIWI domains. PmAgo4 was phylogenetically clustered with the AGO subfamily while exhibited a gonad-specific expression pattern similar to that of proteins in the PIWI subfamily. The expression of PmAgo4 did not change significantly in response to either double-stranded RNA or yellow head virus injection suggesting that PmAgo4 may not be the main AGO proteins that play a role in dsRNA-mediated gene silencing or antiviral defense. Interestingly, PmAgo4 appeared to participate in the control of transposons since the activation of both DNA transposon and retrotransposon was detected in the testis of PmAgo4-knockdown shrimp. Our study thus provided the first evidence for an unusual type of the AGO proteins that was predominantly expressed in shrimp gonad and implication of its role in protecting the shrimp genome against an invasion of transposons. © 2014 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919667479&doi=10.1016%2fj.fsi.2014.11.014&partnerID=40&md5=73fd10e8dc3691c94ed46eb8037c6b74
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Roniviridae
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon
Leebonoi W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
A novel function of bursicon in stimulation of vitellogenin expression in black tiger shrimp, Penaeus monodon Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon (ภาษาอังกฤษ) Functional characterization of a novel C-type lectin gene in the black tiger shrimp, Penaeus monodon Optimisation of electroporation and lipofection protocols to derive the black tiger shrimp cell line (Penaeus monodon) Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp Effects of fatty acids enrichment on polychaetes to be used as a maturation feed for the male black tiger shrimp (Penaeus monodon) A novel localization of molt-inhibiting hormone in the tegumental glands of shrimp Penaeus monodon and its possible role in shrimp molting Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon)
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