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Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum
Krajaejun T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum
ผู้แต่ง / หัวหน้าโครงการ (EN): Krajaejun T.
บทคัดย่อ (EN): Oomycetes form a unique group of microorganisms that share hyphal morphology with fungi. Most of pathogenic oomycetes infect plants, while some species are capable of infecting animals. Pythium insidiosum is the only oomycete that can infect both humans and animals, and causes a life-threatening infectious disease, called 'pythiosis'. Controlling an infection caused by P. insidiosum is problematic because effective antimicrobial drugs are not available. Information on the biology and pathogenesis of P. insidiosum is limited. We generated a P. insidiosum transcriptome of 26735 unigenes, using the 454 sequencing platform. As adaptations to increased temperature inside human hosts are required for a successful pathogen, we generated P. insidiosum transcriptomes at 28°C and 37°C and identified 625 up-regulated and 449 down-regulated genes at 37°C. Comparing the proteomes of oomycetes, fungi, and parasites provided clues on the evolutionary history of P. insidiosum. Potential virulence factors of P. insidiosum, including putative effectors, were identified. Pythium insidiosum harbored an extensive repertoire of ~300 elicitin domain-containing proteins. The transcriptome, presented herein, provides an invaluable resource for exploring P. insidiosum's biology, pathogenesis, and evolution. © 2014 The British Mycological Society.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905216938&doi=10.1016%2fj.funbio.2014.01.009&partnerID=40&md5=27e9dc5ec6ce88ab4fd2e0842da3a0e4
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Virulence Factors
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum
Krajaejun T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
The immunoreactive exo-1,3-β-glucanase from the pathogenic oomycete pythium insidiosum is temperature regulated and exhibits glycoside hydrolase activity The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete pythium insidiosum and evades host antibody responses Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment Biochemical and genetic analyses of the oomycete Pythium insidiosum provide new insights into clinical identification and urease-based evolution of metabolismrelated traits Geographic variation in the elicitin-like glycoprotein, ELI025, of Pythium insidiosum isolated from human and animal subjects Genetic analysis of Thai cattle reveals a Southeast Asian indicine ancestry Whole genomic analysis of bovine group A rotavirus strains A5-10 and A5-13 provides evidence for close evolutionary relationship with human rotaviruses Genome-wide analysis reveals phytohormone action during cassava storage root initiation Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages Comparative genome analysis of fish pathogen Flavobacterium columnare reveals extensive sequence diversity within the species
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