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Multiple diagnostic tests based bio-load and bio-type profiles of mycobacterium avium sub-species paratuberculosis infection in raw milk of buffaloes (Buffalo Bulletin,40(4))
Pradeep Chaudhary - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Multiple diagnostic tests based bio-load and bio-type profiles of mycobacterium avium sub-species paratuberculosis infection in raw milk of buffaloes (Buffalo Bulletin,40(4))
ผู้แต่ง / หัวหน้าโครงการ (EN): Pradeep Chaudhary
หน่วยงานสังกัดผู้แต่ง (EN): Uttar Pradesh (India). Institute of Applied Sciences and Humanities. Department of Biotechnology
บทคัดย่อ (EN): Raw milk samples of 503 individual buffaloes were collected from dairy farms located in Agra and Mathura cities in North India. Multiple tests (Indirect Fluorescent Agglutination test (i_FAT), IS900 PCR, Microscopy, Indigenous ELISA kit (i_ELISA), Dot-ELISA (d_ELISA) and Latex agglutination test (LAT)) based bio-load and bio-type profile of Mycobacterium avium subspecies paratuberculosis (MAP) was studied. Cumulatively average bio-load was 61.2 percent using three antigen and three antibody based. In i_FAT, IS900 PCR and microscopy, 43.5, 13.3 and 40.9 percent milk were positive for MAP, respectively. Whereas, 32.8, 49.3 and 44.1 percent milk samples were positive in i_ELISA, d_ELISA and LAT, respectively. Bio-typing of representative milk samples using IS900 PCR positive raw milk (67), 13.4 percent were infected with 'Indian Bison Type' biotype using IS1311 PCR_REA. Study concluded that 'Indian Bison type' was the predominant bio-type infecting lactating buffaloes of this region. Raw milk was highly convenient sample in buffaloes and 'milk samples' were first time screened without initial processing of milk samples. Detection limits of each tests was improved. Results of five tests (d_ELISA, LAT, i_ELISA, microscopy, i_FAT were comparable, except IS900 PCR. High bio-load of MAP in milk of buffaloes was major health hazard for human health. High bio-load of MAP was alarming and calls for initiation of Johne's disease control programs in the country.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/414287
คำสำคัญ (EN): Indian Bison Type
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Pradeep Chaudhary. "Multiple diagnostic tests based bio-load and bio-type profiles of mycobacterium avium sub-species paratuberculosis infection in raw milk of buffaloes (Buffalo Bulletin,40(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Pradeep Chaudhary. "Multiple diagnostic tests based bio-load and bio-type profiles of mycobacterium avium sub-species paratuberculosis infection in raw milk of buffaloes (Buffalo Bulletin,40(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Multiple diagnostic tests based bio-load and bio-type profiles of mycobacterium avium sub-species paratuberculosis infection in raw milk of buffaloes (Buffalo Bulletin,40(4))
Pradeep Chaudhary
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Comparison of different diagnostic tests for the detection of subclinical mastitis in buffaloes (Buffalo Bulletin,40(4)) Alterations in milk composition due to seasonal and lactational stages in Tarai buffalo (Buffalo Bulletin,40(4)) Histopathology: An old yet important technique to diagnose paratuberculosis in non-descript water buffaloes (Buffalo Bulletin,40(4)) Aquatic fern Azolla affecting milk production in buffaloes (Buffalo Bulletin,41(4)) Effect of type of treated rice straw on ruminal fermentation characteristic and cellilolytic bacteria of swamp buffalo (Buffalo Bulletin,40(4)) Effect of season and stage of lactation on milk components in Purnathadi buffaloes (Buffalo Bulletin,41(3)) Relations of hygiene index scores with milk production in Anatolian buffaloes (Buffalo Bulletin,41(4)) Analysis of export potential of buffalo meat in India (Buffalo Bulletin,40(4)) Metabolic and hormonal changes in water buffaloes during post parturient peak lactation (Buffalo Bulletin,40(4)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1))
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