สืบค้นงานวิจัย
EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS
Rekha - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS
ผู้แต่ง / หัวหน้าโครงการ (EN): Rekha
บทคัดย่อ (EN): Inspite of a few successful events in genetic manipulation, Hevea is still facing the problem of regeneration and hardening of transformants. Use of more juvenile explants may be a solution to address this issue. Multiple embryos /embryogenic calli derived from zygote as the target tissue have been used in many plants for the regeneration of transgenic plants (Dandekar1989). In Hevea, attempts were made to induce embryogenic calli from immature zygotic embryos by adopting in ovulo embryoculture method (Rekha, et.al.2010) coupled with media manipulations. Immature fruits were collected from field grown trees of Hevea, surface sterilized and cultured in the medium. Multiple embryos along with embryogenic calli were induced in Nitsh basal medium supplemented with the growth regulators of zeatin (0.3 mgl-1) along with Gibberellic acid (2 mgl-1) and kinetin (3 mgl-1). Calli were proliferated and used as the target tissue for transformation experiments with three different gene constructs namely Osmotin, hmgr1 and MnSOD. High frequency transformation (70%) could be achieved. Transgenic cell lines were developed and proliferated from all the gene constructs. Successful embryo induction, maturation and plant regeneration were achieved with all the three different gene constructs. Plants have been developed and hardened. Compared to explants of clonal origin, plant regeneration was faster and easier for this explant. The time span for the regeneration of plants could be reduced to 1 year compared to clonal explants which usually takes 2-3 years. The percentage recovery of plants after hardening also could be improved considerably. Regeneration of plants from more events is possible since the system allows easy and rapid plant regeneration and hardening. This inturn enables more effective evaluation and the superior events among the transformants can be identified. Plants developed can be used as breeding materials in hybridization programme. Moreover, this system can be effectively utilized in functional evaluation of the foreign genes inserted. Development of transgenic rootstocks is another possibility of this technique.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): genetic transformation
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Rekha. "EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS". ไม่ระบุหน่วยงาน. ม.ป.ป.

Rekha. "EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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EXPLOITATION OF IN VITRO INDUCED ZYGOTIC POLYEMBRYONY FOR GENETIC TRANSFORMATION IN HEVEA BRASILIENSIS
Rekha
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
GENETIC IMPROVEMENT OF HEVEA BRASILIENSIS IN INDIA Genetic Transformation and Regeneration of Hevea Brasiliensis Transgenic Plant with GAI Gene GENETIC TRANSFORMATION OF HEVEA BRASILIENSIS BY USING A NON-DESTRUCTIVE VISUAL MARKER (GFP) Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis ETHEPON-INDUCED SPECIFIC PROTEINS ON SEVERAL RUBBER PLANT (Hevea Brasiliensis Muell. Arg) Cloning and expression of HbACO2 of Hevea brasiliensis DEVELOPMENT OF DWARF CLONES OF HEVEA BRASILIENSIS CARBOHYDRATE DISTRIBUTION AT TRUNK LEVEL OF HEVEA BRASILIENSIS Plant Hormones and Oxidative Stress in Hevea brasiliensis
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