สืบค้นงานวิจัย
Effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum infected erythrocytes
Saiwaew S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum infected erythrocytes
ผู้แต่ง / หัวหน้าโครงการ (EN): Saiwaew S.
บทคัดย่อ (EN): In severe falciparum malaria cytoadherence of parasitised red blood cells (PRBCs) to vascular endothelium (causing sequestration) and to uninfected red cells (causing rosette formation) contribute to microcirculatory flow obstruction in vital organs. Heparin can reverse the underlying ligand-receptor interactions, but may increase the bleeding risks. As a heparinderived polysaccharide, sevuparin has been designed to retain anti-adhesive properties, while the antithrombin-binding domains have been eliminated, substantially diminishing its anticoagulant activity. Sevuparin has been evaluated recently in patients with uncomplicated falciparum malaria, and is currently investigated in a clinical trial for sickle cell disease. The effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum isolates from Thailand were investigated. Trophozoite stages of P. falciparum-infected RBCs (Pf-iRBCs) were cultured from 49 patients with malaria. Pf-iRBCs were treated with sevuparin at 37?C and assessed in rosetting and in cytoadhesion assays with human dermal microvascular endothelial cells (HDMECs) under static and flow conditions. The proportion of Pf-iRBCs forming rosettes ranged from 6.5% to 26.0% (median = 12.2%). Rosetting was dose dependently disrupted by sevuparin (50% disruption by 250 μg/mL). Overall 57% of P. falciparum isolates bound to HDMECs under static conditions; median (interquartile range) Pf-iRBC binding was 8.5 (3.0-38.0) Pf-iRBCs/1000 HDMECs. Sevuparin in concentrations ô 100 μg/mL inhibited cytoadherence. Sevuparin disrupts P. falciparum rosette formation in a dose dependent manner and inhibits cytoadherence to endothelial cells. The data support assessment of sevuparin as an adjunctive treatment to the standard therapy in severe falciparum malaria. © 2017 Saiwaew et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014432806&doi=10.1371%2fjournal.pone.0172718&partnerID=40&md5=4ce86bb6f4eb7ee159d226ab67b5b4ff
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Trophozoites
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Saiwaew S.. "Effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum infected erythrocytes". ไม่ระบุหน่วยงาน. ม.ป.ป.

Saiwaew S.. "Effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum infected erythrocytes". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum infected erythrocytes
Saiwaew S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Inhibition of merozoite invasion and transient de-sequestration by sevuparin in humans with Plasmodium falciparum malaria Ex-Vivo cytoadherence phenotypes of Plasmodium falciparum strains from Malian children with hemoglobins A, S, and C Artemisinin-resistant Plasmodium falciparum malaria Differential roles of an Anopheline midgut GPI-anchored protein in mediating Plasmodium falciparum and Plasmodium vivax ookinete invasion Evolution of Fseg/Cseg dimorphism in region III of the Plasmodium falciparum eba-175 gene Neutralizing antibodies against Plasmodium falciparum associated with successful cure after drug therapy Targeting the Cell Stress Response of Plasmodium falciparum to Overcome Artemisinin Resistance Quantifying connectivity between local Plasmodium falciparum malaria parasite populations using identity by descent Diversifying selection on the thrombospondin-related adhesive protein (TRAP) gene of plasmodium falciparum in Thailand Combinatorial Genetic Modeling of pfcrt-Mediated Drug Resistance Evolution in Plasmodium falciparum
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