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High-throughput approaches to screen the effective bioplastic producing bacteria by using biodiesel waste by-product as carbon source
Varavut Tanamool - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): High-throughput approaches to screen the effective bioplastic producing bacteria by using biodiesel waste by-product as carbon source
ผู้แต่ง / หัวหน้าโครงการ (EN): Varavut Tanamool
บทคัดย่อ (EN): With dramatic use of biodiesel in world-wild consequently increases the accumulation the huge amounts of by-products, mainly crude glycerol via transesterification reaction. Therefore, use of the biotechnological strategies for biotransformation of crude glycerol to the other valuable products would be advantageous. In this research, we have attempted to use biodiesel waste by-product to produce an environmentally friendly bioplastic of polyhydroxyalkanoates (PHAs) by newly isolated strain from different bacterial sources. The primary isolation was carried out by using the standard morphological and their colonial appearance. 47 positive isolates were screened and selected from 250 isolates by Nile red staining-colony under UV-light. Then, spectrofluorometric technique was used to evaluate the high potential PHAs-producing strain. The results showed that 11 PHAs-producing strains were detected with high folds intensity and effective growth. The accumulation of PHAs was further investigated in cells which were cultured in the medium containing 20 % (w/v) glycerol waste by-product without optimization process and revealed that isolate NK14 showed the highest dry cell weight (6.74 g/L) and PHAs production (3.07 g/L). Therefore, this research results highlight the high potential of microbe that might be the exploitable application for the industrial PHAs production from biodiesel waste by-product.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
จำนวนหน้า: 6
เอกสารแนบ: https://ag2.kku.ac.th/kaj/PDF.cfm?filename=366.pdf&id=2180&keeptrack=15
คำสำคัญ (EN): polyhydroxyalkanoate
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High-throughput approaches to screen the effective bioplastic producing bacteria by using biodiesel waste by-product as carbon source
Varavut Tanamool
ไม่ระบุผู้เผยแพร่
2557
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